高压性静脉管壁胶原和弹力纤维分布与定量研究  

Quantitative analysis and distribution of collagen and elastic fibers in hypertensive vein walls

作　　者：徐永波[1] 赵建华[1] 褚海波[1] 王涛[2] 菅凤国[3] 郭文君[4] 

机构地区：[1]解放军第89医院普外中心,山东潍坊261021 [2]解放军第89医院病理科,山东潍坊261021 [3]昌邑市人民医院普外科,山东昌邑261300 [4]潍坊医学院病理教研室,山东潍坊261042

出　　处：《中国血管外科杂志（电子版）》2013年第2期119-122,共4页Chinese Journal of Vascular Surgery(Electronic Version)

摘　　要：目的探讨高压性脾静脉与曲张大隐静脉管壁中膜胶原和弹力纤维的变化,评估静脉管壁胶原和弹力纤维重塑程度。方法收集门脉高压症脾静脉和曲张大隐静脉管壁标本各20例,另设脾外伤正常脾静脉和正常大隐静脉管壁标本作对照组各20例。行EVG染色,光镜下观察管壁胶原和弹力纤维分布,测定胶原纤维相对含量(RCC)和弹力纤维相对含量(RCE)。结果脾静脉组RCC、RCE分别为(50.75±14.79)%、(11.36±4.03)%,对照组为(31.22±5.71)%、(14.60±2.95)%,RCC、RCE比较差异均有统计学意义(RCC:P<0.01;RCE:P<0.05);曲张组分别为(29.71±6.63)%、(7.50±2.49)%,对照组为(23.52±6.20)%、(9.71±3.89)%,RCC比较差异有统计学意义(P<0.05)。脾静脉组RCC变化比率与曲张组比较有显著性差异(P<0.01)。结论静脉高压状态下脾静脉和大隐静脉管壁中膜胶原纤维含量明显增多、弹力纤维减少,且脾静脉胶原纤维增多比率比大隐静脉更明显。Objective To investigate the changes of collagen and elastic fibers in hypertensive splenic vein and varicose great saphenous vein wall media, in order to compare the degree of collagen and elastic fiber remodeling in the two kinds of veins. Methods Four groups were divided, including Splenic veins of patients with portal hypertension (n=20) and control group (n=20), varicose great saphenous veins (n=20) and control group (n=20) were collected and stained with elastica van Gieson (EVG), the distribution of collagen and elastic fibers in vein walls was observed, and the relative content of collagen (RCC). The relative content of elastic fibers (RCE) were measured with light microscope. Results RCC and RCE were (50.75±14.79)% and (11.36±4.03)%in splenic vein group, (31.22±5.71)% and (14.60±2.95)% in control group, respectively. For RCC and RCE, there were significant differences between the splenic vein group and control group (RCC: P < 0.01 , RCE:P < 0.05). RCC and RCE were (29.71±6.63)% and (7.50±2.49)% in varicose group, (23.52 ± 6.20)% and (9.71 ± 3.89)% in control group, respectively. The difference of RCC was statistically significant (P < 0.05). For change ratio of RCC, there were statistically significant differences between the splenic vein groups and varicose group (P < 0.01). Conclusion Collagen fiber content increased significantly and elastic fibers reduced in splenic vein and great saphenous vein wall media under the state of venous hypertension. And the ratio of increase in splenic veins is significantly more than that in great saphenous veins.

关 键 词：高压性脾静脉 曲张大隐静脉 胶原纤维 弹力纤维 定量分析 

分 类 号：R657.3[医药卫生—外科学]