Endothelin and neonatal capsaicin regulate gastric resistance to injury in BDL rats  

作　　者：Paula RS Cmara Gerson JN Ferraz Licio A Velloso José Murilo R Zeitune Fernando AB Suassuna Jose Geraldo P Ferraz 

机构地区：[1]Department of Medicine, Potiguar University [2]GI Research Lab,Gastrocentro, Department of Medicine, Faculty of Medical Sciences, University of Campinas [3]Division of Gastroenterology, Faculty of Medicine, University of Calgary

出　　处：《World Journal of Gastrointestinal Pathophysiology》2012年第4期85-91,共7页世界胃肠病理生理学杂志（英文版）（电子版）

基　　金：Supported by A fellowship from Fundaao de Amparo a Pes-quisa do Estado de Sao Paulo, FAPESP, Brazil (to Cmara PRS);a research grant from FAPESP (to Ferraz JGP)

摘　　要：AIM: To investigate the relationship between primary afferent neurons, endothelin (ET) and the role of its receptors on ethanol-induced gastric damage in cirrhotic rats. METHODS: Cirrhosis and portal hypertension were induced in rats by bile duct ligation (BDL) while controls had a sham operation. The association between ET and afferent neurons on the gastric mucosa was evaluated by capsaicin treatment in newborn rats, the use of ET agonists or antagonists, gastric ET-1 and -3 mRNA and synthetic capacity. Ethanol-induced damage was assessed using ex vivo gastric chamber experiments.Gastric blood flow was measured by laser-Doppler flow-metry. RESULTS: ET-3 and an ETB receptor antagonist sig- nificantly reduced the extent of ethanol-induced gastric damage in BDL rats. Gastric ET-1 and -3 levels were 30% higher in BDL rats compared to control rats. Cap-saicin treatment restored the gastric resistance and blood flow responses to topical application of ethanol in BDL rats and ET-1 and -3 production to levels observed in controls. CONCLUSION: Our results suggest that the reduced resistance of the gastric mucosa of cirrhotic rats to ethanol-induced injury is a phenomenon modulated by ET through the ET B receptor and by sensory afferent neurons.AIM: To investigate the relationship between primary afferent neurons, endothelin (ET) and the role of its receptors on ethanol-induced gastric damage in cirrhotic rats. METHODS: Cirrhosis and portal hypertension were induced in rats by bile duct ligation (BDL) while controls had a sham operation. The association between ET and afferent neurons on the gastric mucosa was evaluated by capsaicin treatment in newborn rats, the use of ET agonists or antagonists, gastric ET-1 and -3 mRNA and synthetic capacity. Ethanol-induced damage was assessed using ex vivo gastric chamber experiments. Gastric blood flow was measured by laser-Doppler flowmetry. RESULTS: ET-3 and an ETB receptor antagonist significantly reduced the extent of ethanol-induced gastric damage in BDL rats. Gastric ET-1 and -3 levels were 30% higher in BDL rats compared to control rats. Capsaicin treatment restored the gastric resistance and blood flow responses to topical application of ethanol in BDL rats and ET-1 and -3 production to levels observed in controls. CONCLUSION: Our results suggest that the reduced resistance of the gastric mucosa of cirrhotic rats to ethanol-induced injury is a phenomenon modulated by ET through the ETB receptor and by sensory afferent neurons.

关 键 词：ENDOTHELINS TACHYKININS Portal hypertension GASTROPATHY Cirrhosis 

分 类 号：R722.1[医药卫生—儿科]