微柱离心-高效液相色谱法测定莪术醇脂质体的包封率  

Determination of entrapment efficiency of curcumol liposomes by minicolumn centrifugation-high performance liquid chromatography method

作　　者：金鑫[1] 王绛玉 张娜 方亮[1] 李铁福 

机构地区：[1]沈阳药科大学药学院 [2]辽宁中医药大学药学院 [3]沈阳药科大学制药工程学院

出　　处：《中国药剂学杂志（网络版）》2010年第1期27-32,共6页Chinese Journal of Pharmaceutics：Online Edition

基　　金：辽宁省教育厅基金资助项目(20060869)

摘　　要：目的建立莪术醇脂质体包封率测定的微柱离心-高效液相色谱法。方法采用葡聚糖凝胶(Sephadex G-50)微柱离心法分离脂质体和游离药物,采用HPLC法测定脂质体中药物含量,计算包封率。结果葡聚糖凝胶G-50对空白脂质体无吸附,最佳离心条件为3000r·min-1、2min,含量测定的线性范围为2.0～16.0mg·L-1,高、中、低3个质量浓度的日内、日间RSD均小于2%,回收率为99.82%～101.61%,3批样品中莪术醇的平均包封率为73.62%。结论微柱离心-高效液相色谱法可作为莪术醇脂质体质量控制的有效手段之一。Objective To establish a minicolumn centrifugation-high performance liquid chromatography method for determination of entrapment efficiency of curcumol liposomes.Methods The liposomes were separated from free curcumol using Sephadex G-50-filling minicolumn by centrifugation.High performance liquid chromatography was developed to determine the entrapment efficiency of curcumol in liposomes.Results By flowing through the Sephadex G-50 minicolumn,blank liposomes were not absorbed.The condition of centrifugation was 3 000 r·min-1for 2 min.The calibration curve was linear in the range of 2.0-16.0 mg·L-1 for curcumol.The precision of inter-day and intra-day was less than 2 %,and the recoveries of the method were in the range of 99.82 %-101.61 %.Conclusion The method can be used for the quality control of curcumol liposomal formulation.

关 键 词：药剂学 脂质体 微柱离心法 莪术醇 包封率 高效液相色谱法 

分 类 号：R283[医药卫生—中药学]