MDCK-hMDR1细胞测定ML120B的细胞膜渗透性  

Determination of passive permeability of ML120B using MDCK-hMDR1 cell culture

作　　者：张春叶[1] 王东凯[1] 崔文奇[1] 肖玉婷[1] 姚建美[1] 

机构地区：[1]沈阳药科大学药学院

出　　处：《中国药剂学杂志（网络版）》2009年第5期403-408,共6页Chinese Journal of Pharmaceutics：Online Edition

摘　　要：目的测定ML120B的细胞膜渗透性。方法采用MDCK-hMDR1单层膜细胞测定ML120B的体外跨膜转运率及转运机制。结果药物的被动转运渗透性为678.9nm·s-1,不加P-糖蛋白(Pgp)抑制剂的外排率Efflux值为0.8,添加Pgp抑制剂的Efflux值为0.5。结论ML120B属于高渗透性药物,并不是Pgp的作用底物。Objective To determine the passive permeability of ML120B. Methods MDCK-hMDR1 monolayer cells were used to determine the passive permeability of ML120B and its permeation mechanism. Results The passive permeability of ML120B was 678.9 nm·s-1 and the efflux ratio was 0.8 without Pgp inhibitor and 0.5 with Pgp inhibitor. Conclusions ML120B is a highly permeable compound and is not a Pgp substrate.

关 键 词：药剂学 ML120B 渗透性 P-糖蛋白 MDCK-hMDR1 

分 类 号：R96[医药卫生—药理学]