载脂蛋白E基因多态性与冠心病的关联研究  

Association Between Apolipoprotein E genotype And Coronary Heart Disease

作　　者：王学铭[1] 李云贵[1] 曾明[1] 马俊[1] 王吉伟[2] 

机构地区：[1]长沙医学院,长沙市410219 [2]中南大学,长沙市480078

出　　处：《长沙医学院学报》2007年第1期8-10,共3页Journal of Changsha Medical University

基　　金：湖南省教育厅资助课题(04C057)。

摘　　要：目的探讨载脂蛋白E(Apoe)基因多态性对冠心病发病的意义。方法选择30例冠心病患者(CHD组)及30例正常人对照者,应用聚合酶链反应(PCR)—限制性片段长度多态性分析(RFLP)法,以CfoI内切酶及聚丙烯酰胺凝胶电泳确定上述人群中ApoE基因的多态性。结果冠心病组Apoε3/4基因型及ε4等位基因频率都比对照组高,差异有显著性(P<0.01)。结论Apoε3/4等位基因频率升高是CHD发病的重要危险因素。Objective: To explore effect of ApoE genotype polymorphism on CHD morbidity.Methods: ApoE genotype polymorphism of 30 patients with coronary heart disease(CHD group) and 30 healthy(Control group) were determined respectively by using polymerase chain reaction(PCR) with Cfol restriction endonuclease and polyacryloamine gel electrophoresis technology. [Results]:Frequencies of Apo ε 3/4 genotype and ε 4 allele gene in the CHD group were obviously high than that of the control group(p<0.05).Conclusion: We consider that the increasing frequencies of Apo ε 3/4 genotype and ε 4 allele gene may be a CHD harm factor.

关 键 词：冠心病 载脂蛋白E 基因多态性 

分 类 号：R541.4[医药卫生—心血管疾病]