First report on natural Leishmania infection of Phlebotomus sergenti due Leishmania tropica by high resolution melting curve method in Southeastern Iran  

作　　者：Aghaei Afshar A Rassi Y Sharifi I Vatandoost H Mollaie HR Oshaghi M.A Abai MR Rafizadeh S 

机构地区：[1]Leishmaniasis Research Center,Kerman University of Medical Sciences [2]Department of Medical Entomology & Vector Control,School of Public Health,Tehran University of Medical Sciences [3]Department of Medical Virology,Tehran University of Medical Sciences [4]Department of Deputy of Strategic Planning,Reference Health Laboratories Research Center,Deputy of Treatment.Ministry of Health & Medical Education of Health

出　　处：《Asian Pacific Journal of Tropical Medicine》2014年第2期93-96,共4页亚太热带医药杂志（英文版）

基　　金：supported by the Iranian Center of Diseases Management,Ministry of Health and Medical Education;financially supported by School of Public Health,Tehran University of Medical Sciences;Leishmaniasis Research Center,Kerman University of Medical Sciences,project No.10487

摘　　要：Objective:To identify the Leishmania species in infected sand flies by Real-time PCR coupled with HRM analysis.Methods:Real-time PCR coupled with HRM analysis targeting the first internal transcribed spacer(ITS1)of nuclear ribosomal DNA as the genetic marker was used to identify and distinguish Lelthmania species in sand flies specimens.Results:Three out of 115females of Phlebotomus sergenti(P.sergenti)(2.6%)were positive to Leishmania tropica(L.tropica).Conclusions:This is the first report on P.sergenti as the main and proven vector of anthroponitic cutaneous leishmaniasis in Dehbakri County using Real-time PCR coupled with HUM analysis.This method is rapid,sensitive and specific for diagnosing of parasites in infected Sand flies and ideal for large scale genotyping projects.Objective:To identify the Leishmania species in infected sand flies by Real-time PCR coupled with HRM analysis.Methods:Real-time PCR coupled with HRM analysis targeting the first internal transcribed spacer(ITS1)of nuclear ribosomal DNA as the genetic marker was used to identify and distinguish Lelthmania species in sand flies specimens.Results:Three out of 115females of Phlebotomus sergenti(P.sergenti)(2.6%)were positive to Leishmania tropica(L.tropica).Conclusions:This is the first report on P.sergenti as the main and proven vector of anthroponitic cutaneous leishmaniasis in Dehbakri County using Real-time PCR coupled with HUM analysis.This method is rapid,sensitive and specific for diagnosing of parasites in infected Sand flies and ideal for large scale genotyping projects.

关 键 词：Vector(s) LEISHMANIA tropica Real-time PCR Iran 

分 类 号：R757[医药卫生—皮肤病学与性病学]