Design of a multiplex PCR method for detection of toxigenicpathogenic in Vibrio cholerae  

作　　者：Abbas Ali Imani Fooladi Iman Islamieh D Hosseini Doust R Karami A Marashi SM 

机构地区：[1]Applied Microbiology Research Center,Baqiyatallah University of Medical Sciences,Tehran,Iran [2]Department of Microbiology,Faculty of Advance Sciences and Technology,Islamic Azad University-Pharmaceutical Sciences Branch,Tehran,Iran [3]Molecular Biology Research Center,Baqiyatallah University of Medical Sciences,Tehran,Iran [4]Department of Microbiology and Immunology,Babol University of Medical Sciences,Babol,Iran

出　　处：《Asian Pacific Journal of Tropical Medicine》2013年第2期115-118,共4页亚太热带医药杂志（英文版）

摘　　要：Objective:To study virulence and regulatory genes(hlyA,ctxB,tcpI) in clinical strains of Vibrio ckolerae(V.cholerae),simultaneously.Methods:Three important genes,tepI,hlyA and ctxB were used for detection of toxigenic and pathogenic V.cholera by chain reaction assay method. Results:According to the results of the PCR,the incidence of hlyA,tcpI,and ctxB genes in clinical isolates was obtained as 94.7%(72 sample),90.8%(69 sample),and 92.1%(70 sample), respectively.Five strains possessed all genes except ctxB,six strains possessed all genes except tcpI,four strains possessed all genes except hlyA,one strain possessed only hlyA and 60 strains contained a combination of three genes.Including hlyA,ctxB and tcpI,Conclusions:Result show that this method could be reliable to detect toxigenic-pathogenic strains of V.cholerae in Iran.Objective:To study virulence and regulatory genes(hlyA,ctxB,tcpI) in clinical strains of Vibrio ckolerae(V.cholerae),simultaneously.Methods:Three important genes,tepI,hlyA and ctxB were used for detection of toxigenic and pathogenic V.cholera by chain reaction assay method. Results:According to the results of the PCR,the incidence of hlyA,tcpI,and ctxB genes in clinical isolates was obtained as 94.7%(72 sample),90.8%(69 sample),and 92.1%(70 sample), respectively.Five strains possessed all genes except ctxB,six strains possessed all genes except tcpI,four strains possessed all genes except hlyA,one strain possessed only hlyA and 60 strains contained a combination of three genes.Including hlyA,ctxB and tcpI,Conclusions:Result show that this method could be reliable to detect toxigenic-pathogenic strains of V.cholerae in Iran.

关 键 词：VIBRIO CHOLERAE Multiplex PCR RAPID DETECTION 

分 类 号：R378[医药卫生—病原生物学]