A duplex PCR for the rapid and simultaneous detection of Brucella spp.in human blood samples  

作　　者：Reza Mirnejad Mozafar mohamadi Vahbeh Piranfar Seied Mojtaba Mortazavi Reza Kachuei 

机构地区：[1]Molecular Biology Research Center,Baqiyatallah University of Medical Sciences,Tehran,Iran [2]Health Station,Kerman University of Medical Sciences,Kerman,Iran

出　　处：《Asian Pacific Journal of Tropical Medicine》2013年第6期453-456,共4页亚太热带医药杂志（英文版）

基　　金：supported by Molecular Biology Research Center, Baqiyatallah University of Medical Sciences,with grant number BMSU/MBRC-90-001

摘　　要：Objective:To design a duplex PCR for rapid and simultaneous detection of Brucella species, in human blood samples.Methods:Fifty-two peripheral bloods samples were collected from suspicious patients with brucellosis.Following DNA extraction,PCR assay were performed, using three primers that could simultaneously identify and differentiate three major species of pathogenic Brucella in humans and animals.Results:Of the 52 peripheral bloods samples tested, 25 sample(48%) showed positive reactions in PCR.Twelve samples were positive for Brucella abortus(B.abortus)(23%,13 for Brucella melUensis(B.melUensis)(25%) and 0 for Brucella ovis (6.ovis)(Ow.Conclusions:This work de=monstrates dial in case where specific primers were utilized,duplex PCR has proved to be a simple,fast,and relatively inexpensive method for simultaneous detection of important species of Brucella in clinical samples.Objective:To design a duplex PCR for rapid and simultaneous detection of Brucella species, in human blood samples.Methods:Fifty-two peripheral bloods samples were collected from suspicious patients with brucellosis.Following DNA extraction,PCR assay were performed, using three primers that could simultaneously identify and differentiate three major species of pathogenic Brucella in humans and animals.Results:Of the 52 peripheral bloods samples tested, 25 sample(48%) showed positive reactions in PCR.Twelve samples were positive for Brucella abortus(B.abortus)(23%,13 for Brucella melUensis(B.melUensis)(25%) and 0 for Brucella ovis (6.ovis)(Ow.Conclusions:This work de=monstrates dial in case where specific primers were utilized,duplex PCR has proved to be a simple,fast,and relatively inexpensive method for simultaneous detection of important species of Brucella in clinical samples.

关 键 词：BRUCELLA ABORTUS BRUCELLA MELITENSIS BRUCELLA OVIS DUPLEX PCR 

分 类 号：R516.7[医药卫生—内科学]