Acaricidal activity of synthesized titanium dioxide nanoparticles using Calotropis gigantea against Rhipicephalus microplus and Haemaphysalis bispinosa  

作　　者：Sampath Marimuthu Abdul Abdul Rahuman Chidambaram Jayaseelan Arivarasan Vishnu Kirthi Thirunavukkarasu Santhoshkumar Kanayairam Velayutham Asokan Bagavan Chinnaperumal Kamaraj Gandhi Elango Moorthy Iyappan Chinnadurai Siva Loganathan Karthik Kokati Venkata Bhaskara Rao 

机构地区：[1]Unit of Nanotechnology and Bioactive Natural Products,Post Graduate and Research Department of Zoology,CAbdul Hakeem College [2]Environmental Biotechnology Division,School of Bio Sciences and Technology,VIT University

出　　处：《Asian Pacific Journal of Tropical Medicine》2013年第9期682-688,共7页亚太热带医药杂志（英文版）

摘　　要：Objective:To assess the acaricidal activity of titanium dioxide nanoparticles(TiO_2 NPs)synthesized from flower aqueous extract of Calotropis gigantea(C.gigantea)against the larvae of Rhipicephalus(Boophilus)microplus[R.(B.)microplus]and the adult of Haemaphrysalis bispinosa(H.bispinosa).Methods:The lyophilized C.gigantea flower aqueous extract of 50 mg was added with 100 mL of TiO(OH_2)(10 mM)and magnetically stirred for 6 h.Synthesized TiO_2 NPs were characterized by X-ray diffraction(XRD).Fourier transform infrared spectroscopy(FTIR),Scanning electron microscopy(SEM),and Energy dispersive X-ray spectroscopy(EDX).The synthesised TiO_2 NPs were tested against the larvae of R(B.)microplus and adult of H.bispinosa were exposed to filter paper impregnated method.Results:XRD confirmed the crystalline nature of the nanoparticles with the mean size of 10.52 nm.The functional groups for synthesized TiO_2NPs were 1405.19,and 1053.45 cm^(-1)for-NH_2 bending,primary amines and amides and 1053.84and 1078.45 cm^(-1)for C-O.SEM micrographs of the synthesized TiO_2 NPs showed the aggregated and spherical in shape.The maximum efficacy was observed in the aqueous flower extract of C.gigantea and synthesized TiO_2 NPs against R.(B.)microplus(LC_(50)=24.63 and 5.43 mg/L and r^2=0.960 and 0.988)and against H.bispinosa(LC_(50)=35.22 and 9.15 mg/L and r^2=0.969 and 0.969).respectively.Conclusions:The synthesized TiO_2 NPs were highly stable and had significant acaricidal activity against the larvae of R.(B.)microplus and adult of H.bispinosa.This study provides the first report of synthesized TiO_2 NPs and possessed excellent anti-parasitic activity.Objective:To assess the acaricidal activity of titanium dioxide nanoparticles（TiO2 NPs）synthesized from flower aqueous extract of Calotropis gigantea（C.gigantea）against the larvae of Rhipicephalus（Boophilus）microplus[R.（B.）microplus]and the adult of Haemaphrysalis bispinosa（H.bispinosa）.Methods:The lyophilized C.gigantea flower aqueous extract of 50 mg was added with 100 mL of TiO（OH2）（10 mM）and magnetically stirred for 6 h.Synthesized TiO2 NPs were characterized by X-ray diffraction（XRD）.Fourier transform infrared spectroscopy（FTIR）,Scanning electron microscopy（SEM）,and Energy dispersive X-ray spectroscopy（EDX）.The synthesised TiO2 NPs were tested against the larvae of R（B.）microplus and adult of H.bispinosa were exposed to filter paper impregnated method.Results:XRD confirmed the crystalline nature of the nanoparticles with the mean size of 10.52 nm.The functional groups for synthesized TiO2NPs were 1405.19,and 1053.45 cm-1for-NH2 bending,primary amines and amides and 1053.84and 1078.45 cm-1for C-O.SEM micrographs of the synthesized TiO2 NPs showed the aggregated and spherical in shape.The maximum efficacy was observed in the aqueous flower extract of C.gigantea and synthesized TiO2 NPs against R.（B.）microplus(LC50=24.63 and 5.43 mg/L and r2=0.960 and 0.988)and against H.bispinosa(LC50=35.22 and 9.15 mg/L and r2=0.969 and 0.969).respectively.Conclusions:The synthesized TiO2 NPs were highly stable and had significant acaricidal activity against the larvae of R.（B.）microplus and adult of H.bispinosa.This study provides the first report of synthesized TiO2 NPs and possessed excellent anti-parasitic activity.

关 键 词：Rhipicephalus(Boophilus) microplus Haemaphysalis bispinosa Calotropis gigantean TITANIUM dioxide nanoparticles X-ray DIFFRACTION techniques Electron MICROSCOPIC study 

分 类 号：S482.5[农业科学—农药学]