A simple multiplex PCR for assessing prevalence of extended-spectrumβ-lactamases producing Klebsiella pneumoniae in Intensive Care Units of a referral hospital in Shiraz,Iran  

作　　者：Younes Ghasemi Talieh Archin Mohammad Kargar Milad Mohkam 

机构地区：[1]Parmaceutical Sciences Research Center,School of Pharmacy,Shiraz University of Medical Sciences [2]Department of Pharmaceutical Biotechnology,School of Pharmacy,Shiraz University of Medical Sciences [3]Department of Microbiology,Jahrom Branch,Islamic Azad University

出　　处：《Asian Pacific Journal of Tropical Medicine》2013年第9期703-708,共6页亚太热带医药杂志（英文版）

基　　金：supported by Jinan Science and Technology Deyelopment Plans Grant(No.201121040)

摘　　要：Objective:To identify three common genes(bla_(TEM),bla_(SHV)and bla_(CTX-M)responsible for ESBL production in Klebsiella pneumnniae(K.pneumnniae)isolated from Intensive Care Units of Namazi Hospital,Shiraz,Iran.Methods:A total of 60 non-repetitive nosocomial isolates from 60patients were selected during 2009-2010.The phenotypic identification of ESBL production was confirmed by Double Disk Synergy Test(DDST)according to CLSI guidelines.The ESBL's genotype was then analyzed by multiplex PCR of bla_(TEM),bla_(SHV)and bla_(CTX-M)genes and DNA sequencing.Results:The primary susceptibility tests of K.pneumoniae showed that among 10 examined antibiotics,the most resistant and susceptible antibiotics identified in this study were ampicillin and imipenem,respectively.The phenotypic determination of ESBL by DDST showed that 60%(n=36)of isolates produced ESBL.Multiplex PCR of genes among K.pneuunoniae isolates showed that 39%(n=18)of them have TEM,39%in=18)of then have both CTX-M and TEM and 13%(n=8)of them have TEM,SHV,CTX-M.Conclusions:Our findings reveal the high prevalence(60%)of ESBL prcxducing K.pneumnniae from ICL patients along with a new pattern of bla_(TEM)distribution differ from other countries.Objective:To identify three common genes(blaTEM,blaSHVand blaCTX-Mresponsible for ESBL production in Klebsiella pneumnniae（K.pneumnniae）isolated from Intensive Care Units of Namazi Hospital,Shiraz,Iran.Methods:A total of 60 non-repetitive nosocomial isolates from 60patients were selected during 2009-2010.The phenotypic identification of ESBL production was confirmed by Double Disk Synergy Test（DDST）according to CLSI guidelines.The ESBL’s genotype was then analyzed by multiplex PCR of blaTEM,blaSHVand blaCTX-Mgenes and DNA sequencing.Results:The primary susceptibility tests of K.pneumoniae showed that among 10 examined antibiotics,the most resistant and susceptible antibiotics identified in this study were ampicillin and imipenem,respectively.The phenotypic determination of ESBL by DDST showed that 60%（n=36）of isolates produced ESBL.Multiplex PCR of genes among K.pneuunoniae isolates showed that 39%（n=18）of them have TEM,39%in=18)of then have both CTX-M and TEM and 13%（n=8）of them have TEM,SHV,CTX-M.Conclusions:Our findings reveal the high prevalence（60%）of ESBL prcxducing K.pneumnniae from ICL patients along with a new pattern of blaTEMdistribution differ from other countries.

关 键 词：KLEBSIELLA PNEUMONIAE ESBLs ICUS Multiplex PCR 

分 类 号：R563.1[医药卫生—呼吸系统]