Larvicidal efficacy of Catharanthus roseus Linn.(Family:Apocynaceae)leaf extract and bacterial insecticide Bacillus thuringiensis against Anopheles stephensi Liston.  

作　　者：Chellasamy Panneerselvam Kadarkarai Murugan Kalimuthu Kovendan Palanisamy Mahesh Kumar Sekar Ponarulselvam Duraisamy Amerasan Jayapal Subramaniam Jiang-Shiou Hwang 

机构地区：[1]DRDO-BU Center for Life Sciences,Bharathiar University,Coimhatore 641046,Tamil Nadu,India [2]Division of Entomology,Department of Zoology,School of Life Sciences,Bharathiar University,Coimbatore 641046,Tamil Nadu,India [3]Institute of Marine Biology,National Taiwan Ocean University,Keelung 202-24,Taiwan

出　　处：《Asian Pacific Journal of Tropical Medicine》2013年第11期847-853,共7页亚太热带医药杂志（英文版）

摘　　要：Objective:To explore the larvicidal activity of Catharanthus roseus(C.roseus) leaf extract and Raeillus thuringiensis(B.thuringiensis) against the malarial vector Anopheles stephensi(An. stephensi),when being used alone or together.Methods:The larvicidal activity was assayed at various concentrations under the laboratory and field conditions.The LC_(50) and LC_(90) values of the C.roseus leaf extract were determined by probit analysis.Results:The plant extract showed larvicidal effects after 24 h of exposure;however,the highest larval mortality was found in the petroleum ether extract of C.roseus against the first to fourth instars larvae with LC_(50)=3.34,4.48, 5.90 and 8.17 g/L,respectively;B.thuringiensis against the first to fourth instars larvae with LC_(50)=1.72.1.93.2.17 and 2.42 g/L.respectively:and the combined treatment with LC_(50)=2.18.2.41. 2.76 and 3.22 g/L,respectively.No mortality was observed in the control.Conclusions:The petroleum ether extract of C.roseus extract and B.thuringiensis have potential to be used as ideal eco-friendly agents for the control of An.stephensi in vector control programs.The combined treatment with this plant crude extract and bacterial toxin has better larvicidal efficacy against An.stephensi.Objective:To explore the larvicidal activity of Catharanthus roseus（C.roseus） leaf extract and Raeillus thuringiensis（B.thuringiensis） against the malarial vector Anopheles stephensi（An. stephensi）,when being used alone or together.Methods:The larvicidal activity was assayed at various concentrations under the laboratory and field conditions.The LC50 and LC90 values of the C.roseus leaf extract were determined by probit analysis.Results:The plant extract showed larvicidal effects after 24 h of exposure;however,the highest larval mortality was found in the petroleum ether extract of C.roseus against the first to fourth instars larvae with LC50=3.34,4.48, 5.90 and 8.17 g/L,respectively;B.thuringiensis against the first to fourth instars larvae with LC50=1.72.1.93.2.17 and 2.42 g/L.respectively:and the combined treatment with LC50=2.18.2.41. 2.76 and 3.22 g/L,respectively.No mortality was observed in the control.Conclusions:The petroleum ether extract of C.roseus extract and B.thuringiensis have potential to be used as ideal eco-friendly agents for the control of An.stephensi in vector control programs.The combined treatment with this plant crude extract and bacterial toxin has better larvicidal efficacy against An.stephensi.

关 键 词：CATHARANTHUS roseus Bacillus THURINGIENSIS Anopheles STEPHENSI LARVICIDAL activity 

分 类 号：R184[医药卫生—流行病学]