Apoptosis,antimicrobial and antioxidant activities of phytochemicals from Garcinia malaccensis Hk.f  

作　　者：Muhammad Taher Deny Susanti Mohamad Fazlin Rezali Farah Syahidah Ahmad Zohri Solachuddin Jauhari Arief Ichwan Suhaib Ibrahim Alkhamaiseh Farediah Ahmad 

机构地区：[1]Department of Pharmaceutical Technology,Kulliyyah of Pharmacy,International Islamic University Malaysia,Jalan Istana,Bandar Indera Mahkota,25200 Kuantan,Pahang,Malaysia [2]Department of Biomedical Science,Kulliyyah of Science,International Islamic University Malaysia,Jalan Istana,Bandar Indera Mahkota,25200 Kuantan,Pahang,Malaysia [3]SIRIM Rerhad(National Metrology Laboratory),Lot PT 4803,Bandar Baru Salak Tinggi,43900 Sepang,Selangor,Malaysia [4]Kulliyyah of Dentistry,International Islamic University Malaysia,Jalan Istana,Bandar Indera Mahkota,25200 Kuantan,Pahang,Malaysia [5]Department of Chemistry,Faculty of Science,University Teknologi Malaysia,81310 UTM,Johor,Malaysia

出　　处：《Asian Pacific Journal of Tropical Medicine》2012年第2期136-141,共6页亚太热带医药杂志（英文版）

基　　金：partially supported by the Intermtional Islamic University Malaysia(HUM)through EDWB1002-350

摘　　要：Objective:To study the chemical constituents of stembark of Garcinia malaccenm（G.malaccenm） together with apoptotic.antimicrobial and antioxidant activities.Methods:Purification and structure elucidation were carried out by chromatographic and spectroscopic techniques, respectively.MTT and trypan blue exclusion methods were performed to study the cytotoxic activity.Antibacterial activity was conducted by dise diffusion and microdilulion methods, whereas antioxidant activities were done by ferric thiocyanate method and DPPH radical scavenging.Results:The phylochemical study led lo the isolation ofα,β-mangostin and cycloarl-24-en-3β-ol.α-Mangostin exhibited cytotoxic activity against HSC-3 cells with an IC₅₀ of 0.33μM.β- andα-mangostin showed activity against K562 cells with IC₅₀ of 0.40μM and 0.48μM,respectively,α-Mangostin was active against Gram-positive bacteria, Staphylococcus aureus（S.aureus） and Bacilus anthracis（B.anthmcis） with inhibition zone and MIC value of（19 mm;0.02S mg/mL） and（20 mm;0.013 mg/mL）,respectively.In antioxidant assay,α-mangostin exhibited activity as an inhibitor of lipid peroxidation.Conclusions:G.malaccenm presenceα- andβ-mangostin and cycloart-24-en-3β-ol.β-Mangostin was found very active against H.SC-3 cells and KS62.The results suggest that mangoslins derivatives have the potential to inhibit the growth of cancer cells by inducing apoptosis.In addition,α-andβ-mangostin was found inhibit the growth of Cram-positive pathogenic bacteria and also showed the activity as an inhibitor of lipid peroxidation.Objective:To study the chemical constituents of stembark of Garcinia malaccenm(G.malaccenm) together with apoptotic.antimicrobial and antioxidant activities.Methods:Purification and structure elucidation were carried out by chromatographic and spectroscopic techniques, respectively.MTT and trypan blue exclusion methods were performed to study the cytotoxic activity.Antibacterial activity was conducted by dise diffusion and microdilulion methods, whereas antioxidant activities were done by ferric thiocyanate method and DPPH radical scavenging.Results:The phylochemical study led lo the isolation ofα,β-mangostin and cycloarl-24-en-3β-ol.α-Mangostin exhibited cytotoxic activity against HSC-3 cells with an IC_(50) of 0.33μM.β- andα-mangostin showed activity against K562 cells with IC_(50) of 0.40μM and 0.48μM,respectively,α-Mangostin was active against Gram-positive bacteria, Staphylococcus aureus(S.aureus) and Bacilus anthracis(B.anthmcis) with inhibition zone and MIC value of(19 mm;0.02S mg/mL) and(20 mm;0.013 mg/mL),respectively.In antioxidant assay,α-mangostin exhibited activity as an inhibitor of lipid peroxidation.Conclusions:G.malaccenm presenceα- andβ-mangostin and cycloart-24-en-3β-ol.β-Mangostin was found very active against H.SC-3 cells and KS62.The results suggest that mangoslins derivatives have the potential to inhibit the growth of cancer cells by inducing apoptosis.In addition,α-andβ-mangostin was found inhibit the growth of Cram-positive pathogenic bacteria and also showed the activity as an inhibitor of lipid peroxidation.

关 键 词：GARCINIA malaccensis GUTTIFERAE APOPTOSIS Antibacterial ANTIOXIDANT 

分 类 号：R285[医药卫生—中药学]