Mosquito larvicidal properties of Orthosiphon thymiflorus(Roth)Sleesen.(Family:Labiatae)against mosquito vectors,Anopheles stephensi,Culex quinquefasciatus and Aedes aegypti(Diptera:Culicidae)  

作　　者：K Kovendan K Murugan S Vincent Donald R Barnard 

机构地区：[1]Division of Entomology,Department of Zoology,School of Life Sciences,Bharathiar University,Coimbatore-641046,Tamil Nadu,India [2]P.G.Research & Department of Advanced Zoology and Biotechnology,Loyola College,Nungambakkam,Chennai-600 034,Tamil Nadu,India [3]Center for Medical,Agricultural,and Veterinary Entomology,USDA-ARS,1600 SW 23rd Drive,Gainesville,FL 32608,USA

出　　处：《Asian Pacific Journal of Tropical Medicine》2012年第4期299-305,共7页亚太热带医药杂志（英文版）

基　　金：the Department of Science and Technology(DST),Govt.of India New Delhi,India;Tamil Nadu State Council for Science and Technology(TNSCST), Chennai,Tamil Nadu for providing financial support for the present work

摘　　要：Objective:To determine the mosquito larvicidal activities of hexane,chloroform,ethyl acetate, acetone and methanol leaf extract of Orthosiphon thymiflorus（O.thymiflorus） against Anopheles stephensi（An.stephensi）,Culex quinquefasciatus（Cx.quinquefasciatus） and Aedes aegypti （Ae.aegypti）.Methods:The larvicidal activity was assayed against three mosquito species at various concentrations ranging from（50-450 ppm） under the laboratory conditions.The LC₅₀ and LC₉₀value of the O.thymiflorus leaf extract was determined by Probit analysis.Results:The LC₅₀ values of hexane,chloroform,ethyl acetate,acetone and methanol extract of O.thymiflorus third instar larvae of An.stephensi were LC₅₀= 201.39,178.76,158.06,139.22 and 118.74 ppm; Cx.quinquefasciatus were LC₅₀=228.13,209.72.183.35,163.55 and 149.96 ppm and Ae.aegypti were LC₅₀=215.65,197.91,175.05,154.80 and 137.26 ppm,respectively.Maximum larvicidal activity was observed in the methanolic extract followed by acetone,ethyl acetate chloroform and hexane extract.The larval mortality was observed after 24 h exposure.No mortality was observed in control.Conclusions:The present results suggest that the effective plant crude extracts have potential to be used as an ideal eco-friendly approach for the control of mosquito vectors.This study provides the first report on the larvicidal activity of this plant crude solvent extract of against An.stephensi,Cx.quinquefasciatus and Ae.aegypti mosquitoes.Objective:To determine the mosquito larvicidal activities of hexane,chloroform,ethyl acetate, acetone and methanol leaf extract of Orthosiphon thymiflorus(O.thymiflorus) against Anopheles stephensi(An.stephensi),Culex quinquefasciatus(Cx.quinquefasciatus) and Aedes aegypti (Ae.aegypti).Methods:The larvicidal activity was assayed against three mosquito species at various concentrations ranging from(50-450 ppm) under the laboratory conditions.The LC_(50) and LC_(90)value of the O.thymiflorus leaf extract was determined by Probit analysis.Results:The LC_(50) values of hexane,chloroform,ethyl acetate,acetone and methanol extract of O.thymiflorus third instar larvae of An.stephensi were LC_(50)= 201.39,178.76,158.06,139.22 and 118.74 ppm; Cx.quinquefasciatus were LC_(50)=228.13,209.72.183.35,163.55 and 149.96 ppm and Ae.aegypti were LC_(50)=215.65,197.91,175.05,154.80 and 137.26 ppm,respectively.Maximum larvicidal activity was observed in the methanolic extract followed by acetone,ethyl acetate chloroform and hexane extract.The larval mortality was observed after 24 h exposure.No mortality was observed in control.Conclusions:The present results suggest that the effective plant crude extracts have potential to be used as an ideal eco-friendly approach for the control of mosquito vectors.This study provides the first report on the larvicidal activity of this plant crude solvent extract of against An.stephensi,Cx.quinquefasciatus and Ae.aegypti mosquitoes.

关 键 词：Orthosiphon thymiflorus Anopheles STEPHENSI AEDES aegypti CULEX quinquefasciatus LARVICIDAL activity 

分 类 号：R184[医药卫生—流行病学]