Inflammatory mediator release by Brugia malayi from macrophages of susceptible host Mastomys coucha and THP-1 and RAW 264.7 cell lines  

作　　者：Shiv Kumar Verma Vikas Kushwaha Vijaya Dubey Kirti Saxena Aakanksha Sharma Puvvada Kalpana Murthy 

机构地区：[1]Division of Parasitology,Central Drug Research Institute,CSIR,Lucknow 226001,India

出　　处：《Asian Pacific Journal of Tropical Medicine》2011年第2期92-96,共5页亚太热带医药杂志（英文版）

基　　金：supported by a grant Indian Council of Medical Research, New Delhi and SP/SO/B-46/2000 from the Department of Science and Technology,New Delhi;UGC Senior Research Fellowship support to SKV

摘　　要：Objective:To investigate which life stage of the parasite has the ability to stimulate release of pro- or anti-inflammatory mediators from macrophages.Methods:The human macrophage/ monocyte cell line THP-1,the mouse macrophage cell line RAW 264.7 and naive peritoneal macrophages（PM） from the rodent host Mastomys coucha（M.coucha） were incubated at 37℃in 5%CO₂ atmosphere with extracts of microfilariae（Mf）,third stage infective larvae（L₃） and adult worms（Ad） of Brugia malayi.After 48 hr post exposure,IL-1β,IL-6,TNF-α,IL-10 and nitric oxide（NO） in cell-free supernatants were estimated.Results:Extracts of all the life stages of the parasite were capable of stimulating pro-（IL-1β,IL-6 and TNF-α） and anti-inflammatory （IL-10） cytokines in both the cell lines and peritoneal macrophages of M.coucha.Mf was the strongest stimulator of pro-inflammatory cytokines followed by L₃ and Ad;however,Ad was a strong stimulator of IL-10 release.Mf was found to have potential to modulate LPS-induced NO release in RAW cells.Ad-induced NO release was concentration dependent with maximum at 20μg/mL in both RAW and PMs.Conclusions:The results show that parasites at all life stages were capable of stimulating pro-（IL-1β,IL-6 and TNF-α） and anti-inflammatory（IL-10） cytokines and NO release from macrophages of susceptible host M.coucha,human and mouse macrophage cell lines.Mf can suppress the LPS-induced NO release in RAW cells.The findings also show that the two cell lines may provide a convenient in vitro system for assaying parasite-induced inflammatory mediator release.Objective:To investigate which life stage of the parasite has the ability to stimulate release of pro- or anti-inflammatory mediators from macrophages.Methods:The human macrophage/ monocyte cell line THP-1,the mouse macrophage cell line RAW 264.7 and naive peritoneal macrophages(PM) from the rodent host Mastomys coucha(M.coucha) were incubated at 37℃in 5%CO_2 atmosphere with extracts of microfilariae(Mf),third stage infective larvae(L_3) and adult worms(Ad) of Brugia malayi.After 48 hr post exposure,IL-1β,IL-6,TNF-α,IL-10 and nitric oxide(NO) in cell-free supernatants were estimated.Results:Extracts of all the life stages of the parasite were capable of stimulating pro-(IL-1β,IL-6 and TNF-α) and anti-inflammatory (IL-10) cytokines in both the cell lines and peritoneal macrophages of M.coucha.Mf was the strongest stimulator of pro-inflammatory cytokines followed by L_3 and Ad;however,Ad was a strong stimulator of IL-10 release.Mf was found to have potential to modulate LPS-induced NO release in RAW cells.Ad-induced NO release was concentration dependent with maximum at 20μg/mL in both RAW and PMs.Conclusions:The results show that parasites at all life stages were capable of stimulating pro-(IL-1β,IL-6 and TNF-α) and anti-inflammatory(IL-10) cytokines and NO release from macrophages of susceptible host M.coucha,human and mouse macrophage cell lines.Mf can suppress the LPS-induced NO release in RAW cells.The findings also show that the two cell lines may provide a convenient in vitro system for assaying parasite-induced inflammatory mediator release.

关 键 词：Cytokines Macrophage ThP-1 RAW 264.7 BRUGIA malayi INFECTIVE larva MICROFILARIA Adult worm MASTOMYS coucha 

分 类 号：R532.15[医药卫生—内科学]