Antimicrobial,antiplasmodial,haemolytic and antioxidant activities of crude extracts from three selected Togolese medicinal plants  

作　　者：Simplice D Karou Tchadjobo Tchacondo Lassina Ouattara Kokou Anani Aly Savadogo Amegnona Agbonon Mossaclok Ben Attaia Comlan de Souza Mohsen Sakly Jacques Simpore 

机构地区：[1]Centre de Recherche et de Formation sur les Plantes Medicinales(CERFOPLAM),Universite de Lome,Togo [2]Centre de Recherche Biomoleculaire Pietro Annigoni(CERBA/LABIOGENE),Ouagadougou,Burkina Faso [3]Centre de Recherche en Sciences Biologiques,Alimentaires et Nutritionnelles(CRSBAN),Universite de Ouagadougou,Burkina Faso [4]Laboratoire de Biosurveillance de l'Environnement,Facultes des Sciences de Bizerte,Universite 7 Novembre a Carthage,Tunis,Tunisie

出　　处：《Asian Pacific Journal of Tropical Medicine》2011年第10期808-813,共6页亚太热带医药杂志（英文版）

摘　　要：Objective:To investigate the antioxidant,antimicrobial,antiplasmodial,acute toxicity and haemolytic activities of methanolic extracts of three plants.Phytochemical analysis to determine the phenolic contents was also carried out.Methods:The 2,2-diphenyl-1- picryl-hydrazyl（DPPH） free radical scavenging,NCCLS broth microdilution and Plasmodium Lactate Dehydrogenase（pLDH） assays were used to determine antioxidant,antimicrobial and antiplasmodial activities,respectively.Haemolysis assay was conducted on A⁺ human red blood cells and acute toxicity on male Swiss albino mice.Phenolics were quantitatively determined using spectrophotometric methods.Results:The DPPH assay yielded interesting antioxidant activities of methanolic extract of Parinari curatellifolia（P.curatellifolia） and Entada africana （E.africana）(IC₅₀ were 0.20±0.01μg/mL and 0.47±0.01μg/mL,respectively).This activity was highly correlated with phenolic contents of extracts.The antimicrobial tests displayed minimal inhibitory concentrations（MICs） values ranging from 0.90 to 1.80 mg/mL for Serratia marcescens （5.marcescens） the most susceptible bacterial strain.MIC value was 1.20 mg/mL for susceptible fungal strains including Mucor rouxi（M.rouxi）,Fusarium oxyporum（F.oxyporum） and Rhizopus nigricans（R.nigricans）.pLDH assay showed moderate antiplasmodial activity of Balanites aegyptiaca（B.aegyptiaca）(IC₅₀= 24.56±3.45μg/mL),however this extract was highly haemolytic and toxic in mice(LD₅₀= 625±128mg/kg).Conclusions:Our results support in part the use of the selected plants in the treatment of microbial infections.In addition the plant showed interesting antioxidant activity that could be useful in the management of oxidative stress.Objective:To investigate the antioxidant,antimicrobial,antiplasmodial,acute toxicity and haemolytic activities of methanolic extracts of three plants.Phytochemical analysis to determine the phenolic contents was also carried out.Methods:The 2,2-diphenyl-1- picryl-hydrazyl(DPPH) free radical scavenging,NCCLS broth microdilution and Plasmodium Lactate Dehydrogenase(pLDH) assays were used to determine antioxidant,antimicrobial and antiplasmodial activities,respectively.Haemolysis assay was conducted on A^+ human red blood cells and acute toxicity on male Swiss albino mice.Phenolics were quantitatively determined using spectrophotometric methods.Results:The DPPH assay yielded interesting antioxidant activities of methanolic extract of Parinari curatellifolia(P.curatellifolia) and Entada africana (E.africana)(IC_(50) were 0.20±0.01μg/mL and 0.47±0.01μg/mL,respectively).This activity was highly correlated with phenolic contents of extracts.The antimicrobial tests displayed minimal inhibitory concentrations(MICs) values ranging from 0.90 to 1.80 mg/mL for Serratia marcescens (5.marcescens) the most susceptible bacterial strain.MIC value was 1.20 mg/mL for susceptible fungal strains including Mucor rouxi(M.rouxi),Fusarium oxyporum(F.oxyporum) and Rhizopus nigricans(R.nigricans).pLDH assay showed moderate antiplasmodial activity of Balanites aegyptiaca(B.aegyptiaca)(IC_(50)= 24.56±3.45μg/mL),however this extract was highly haemolytic and toxic in mice(LD_(50)= 625±128mg/kg).Conclusions:Our results support in part the use of the selected plants in the treatment of microbial infections.In addition the plant showed interesting antioxidant activity that could be useful in the management of oxidative stress.

关 键 词：ANTIOXIDANT ANTIMICROBIAL Phenolics HAEMOLYTIC 

分 类 号：R285[医药卫生—中药学]