High prevalence of pathogenic Leptospira in wild and domesticated animals in an endemic area of China  

作　　者：Wang Yalin Zeng Lingbing Yang Hongliang Xu Jianmin Zhang Xiangyan Guo Xiaokui Pal Utpal Qin Jinhong 

机构地区：[1]Department of Medical Microbiology and Parasitology,Institutes of Medical Sciences,Shanghai Jiao Tong University School of Medicine [2]Department of Microbiology,Immunology and Pathology,Colorado State University,Fort Collins,CO,USA 80526 [3]Jiangxi Provincial Center for Disease Control and Prevention [4]Department of Veterinary Medicine,University of Maryland,College Park,Maryland,20742,USA

出　　处：《Asian Pacific Journal of Tropical Medicine》2011年第11期841-845,共5页亚太热带医药杂志（英文版）

基　　金：supported by grants from,the National Natural Science Foundation of China(30970125,81101264 and 811715871);the Program of Shanghai Health Bureau(2008045)

摘　　要：Objective:To assess the prevalence of Leptospira detected in wildlife and domesticated animals in Jiangxi Province,China,in 2009.Methods:Urine samples from 28 buffaloes and kidney samples from 50 pigs,50 dogs and 38 rats were collected from Fuliang and Shangrao County,Jiangxi Province,China,in October 2009.Polymerase chain reaction(PCR) and culture analyses were used to detect Leptospira.The cultured isolates were typed using the microscopic agglutination lest(MAT).Results:The results showed that rats potentially serve as the main reservoir of leptospiral infection,followed by dogs.Although 16%of rats(6/38) were positive using culture analysis,PCR analysis using the diagnostic primers G1/G2 and B64Ⅰ/B64Ⅱor lipLS2 showed identification as 50%and 24%,respectively,of the rat samples as positive for the presence of leptospiral DNA.Conclusions:PCR-based detection of leptospiral DNA in infected kidney tissues of reservoirs is more efficient when using G1/G2 primers than lipL32 primers. However,the latter primers have a potential application for detection in urine samples.The alarmingly high prevalence of leptospiral DMA in the wild rat population near human habitation underscores the utility of routine Leptospira surveillance,preferably using PCR methods,which are more sensitive than traditional culture-based methods.Objective:To assess the prevalence of Leptospira detected in wildlife and domesticated animals in Jiangxi Province,China,in 2009.Methods:Urine samples from 28 buffaloes and kidney samples from 50 pigs,50 dogs and 38 rats were collected from Fuliang and Shangrao County,Jiangxi Province,China,in October 2009.Polymerase chain reaction(PCR) and culture analyses were used to detect Leptospira.The cultured isolates were typed using the microscopic agglutination lest(MAT).Results:The results showed that rats potentially serve as the main reservoir of leptospiral infection,followed by dogs.Although 16%of rats(6/38) were positive using culture analysis,PCR analysis using the diagnostic primers G1/G2 and B64Ⅰ/B64Ⅱor lipLS2 showed identification as 50%and 24%,respectively,of the rat samples as positive for the presence of leptospiral DNA.Conclusions:PCR-based detection of leptospiral DNA in infected kidney tissues of reservoirs is more efficient when using G1/G2 primers than lipL32 primers. However,the latter primers have a potential application for detection in urine samples.The alarmingly high prevalence of leptospiral DMA in the wild rat population near human habitation underscores the utility of routine Leptospira surveillance,preferably using PCR methods,which are more sensitive than traditional culture-based methods.

关 键 词：LEPTOSPIROSIS PREVALENCE China PCR Culture Reservoir 

分 类 号：R531[医药卫生—内科学]