Detection of non-typhoid Salmonella infection by citrus and citrus extracts in Lao PDR  

作　　者：Yutaka Midorikawa Satoshi Nakamura Rattanaphone Phetsouvanh Manivanh Vongsouvaht Kaoru Midorikawa 

机构地区：[1]Suzuka,University Medical Science [2]Research,Institute of National Center for Global Health and Medicine [3]Wellcome,Trust-Mahosot Hospital Oxford University Tropical Medicine Research Collaboration [4]Department of Environmental and Molecular Medicine,Mie University Graduate School of Medicine

出　　处：《Asian Pacific Journal of Tropical Medicine》2010年第12期939-942,共4页亚太热带医药杂志（英文版）

基　　金：supported by 'The development and practical use of a device for the simple detection method of Salmonella by a citrus fruit extraction(Issue number 18650222 2006-2008)';'Elucidaation of mechanism and dissemination of new detection methods by using citrus extracts for food poisoning bacteria such as Salmonella (Issue number 22500783 2010-2012)' ;'International Cooperation Research concerning water-borne diseases in relocated people and the development of related risk management techniques(Issue number 2256003 0001 2010- 2012)' grant aid of Japanese Ministry of Education,Culture. Sports,Science and Technology

摘　　要：Objective:To know the current state of non-typhoid Salmonella infection in Laos.To examine the usefulness of new screening methods for Salmonella using citrus.Methods:Non-typhoid Salmonella infection of person in Lao PDR was studied in this research(2004-2009).The site was Vientiane capital city in 2004.Research from rural villages locating suburb of Vientiane during 2005-2008 was carried out.Rural villages in Attapu province where ethnic minorities were living was searched for this study in 2009.During this research,to detect Salmonella strain, a new method using citrus and citrus extract named MY phenomenon that observing black ring (MIDO ring) on DHL agar was tried.The slice lemon and lime were used for this trial in 2004. After 2005,disk of ascorbic acid and citric acid were used for the device instead of citrus fruits itself.Results:During this research,65 of 272 human samples(23.9%) were infected with non- typhoid Salmonella.Conclusions:During this study,the method using citrus and citrus extracts was accepted for the detection of Salmonella.This study shows that with citrus and citrus extract, detection of Salmonella is possible using only DHL media.Results suggest that infectious rate of non-typhoid Salmonella was high.Objective:To know the current state of non-typhoid Salmonella infection in Laos.To examine the usefulness of new screening methods for Salmonella using citrus.Methods:Non-typhoid Salmonella infection of person in Lao PDR was studied in this research(2004-2009).The site was Vientiane capital city in 2004.Research from rural villages locating suburb of Vientiane during 2005-2008 was carried out.Rural villages in Attapu province where ethnic minorities were living was searched for this study in 2009.During this research,to detect Salmonella strain, a new method using citrus and citrus extract named MY phenomenon that observing black ring (MIDO ring) on DHL agar was tried.The slice lemon and lime were used for this trial in 2004. After 2005,disk of ascorbic acid and citric acid were used for the device instead of citrus fruits itself.Results:During this research,65 of 272 human samples(23.9%) were infected with non- typhoid Salmonella.Conclusions:During this study,the method using citrus and citrus extracts was accepted for the detection of Salmonella.This study shows that with citrus and citrus extract, detection of Salmonella is possible using only DHL media.Results suggest that infectious rate of non-typhoid Salmonella was high.

关 键 词：Non-typhoid SALMONELLA CITRUS MY phenomenon MIDO ring Lao PDR 

分 类 号：R285[医药卫生—中药学]