In vitro-in vivo studies on anti-trypanosomal potentials of Zapoteca portoricensis  

作　　者：Nwodo NJ Omeje EO Bran R 

机构地区：[1]Department of Pharmaceutical Chemistry,University of Nigeria,Nsukka,Nigeria [2]Department of Medical Parasitology and Infection Biology,Swiss Tropical Institute,CH-4002 Basel,Switzerland

出　　处：《Asian Pacific Journal of Tropical Medicine》2009年第1期25-29,共5页亚太热带医药杂志（英文版）

摘　　要：Objective:Aqueous extracts of Zapoteca portoricensis are used traditionally as antidiarrhea agent and in the treatment of diverse gastrointestinal disorders here in Nigeria specifically,the southern part.Similarly,the aqueous extract of the plant is also used traditionally as anticonvulsant,antispasmodic and in the treatment of tonsillitis.Recently too,the anti-inflammatory and antimicrobial activities of the methanol extracts of the root of Zapoteca portoricensis was reported.In this research,we are set to investigate the trypanocidal activity of Zapoteca portoricensis.Methods:The methanol extract of the root of Zapoteca portoricensis was investigated for both in vitro and in vivo trypanocidal activity following established models.In summary,phytochemical analysis was carried out on both the crude powdered root and on the methanol extract following standard procedures. The oral acute toxicity test(LD₅₀ ) of the crude methanol extract was determined according to the method described by Lorke（1983）.Albino mice（17g-21g） of either sex were used.The methanol extract was suspended in 3%v/v tween 85 and administered orally at doses of 10 mg/kg,100 mg/kg and 1 000 mg/kg to three groups of mice（n = 3 ）.The animals were observed for 24 hours.Based on the result obtained in this initial test,doses of 4 mg/kg,6 mg/kg,and 8 mg/kg were administered to three different mice.The LD₅₀ was calculated as the geometric mean of the lowest dose killing a mouse and the highest dose showing no death.The invivo /in-vitro antitrypanosomal evaluations were carried out in experimental animals and tissue cell culture respectively. Results:The result of the in vitro studies shows the inhibitive concentration-50（IC-50） against Trypanosoma brucei rhodesiense（T.b.rhodesiense） to be 0.372 mg/kg,while the control drug melarsoprol was 0.006 mg/kg.On Trypanosoma brucei brucei（T.cruzi）,the IC-50 is 6.42 mg/kg against 0.87 of the reference drug Benznidazole.The cytotoxicity on L-6 cells exhibited an IC-50 Objective:Aqueous extracts of Zapoteca portoricensis are used traditionally as antidiarrhea agent and in the treatment of diverse gastrointestinal disorders here in Nigeria specifically,the southern part.Similarly,the aqueous extract of the plant is also used traditionally as anticonvulsant,antispasmodic and in the treatment of tonsillitis.Recently too,the anti-inflammatory and antimicrobial activities of the methanol extracts of the root of Zapoteca portoricensis was reported.In this research,we are set to investigate the trypanocidal activity of Zapoteca portoricensis.Methods:The methanol extract of the root of Zapoteca portoricensis was investigated for both in vitro and in vivo trypanocidal activity following established models.In summary,phytochemical analysis was carried out on both the crude powdered root and on the methanol extract following standard procedures. The oral acute toxicity test(LD_(50) ) of the crude methanol extract was determined according to the method described by Lorke(1983).Albino mice(17g-21g) of either sex were used.The methanol extract was suspended in 3%v/v tween 85 and administered orally at doses of 10 mg/kg,100 mg/kg and 1 000 mg/kg to three groups of mice(n = 3 ).The animals were observed for 24 hours.Based on the result obtained in this initial test,doses of 4 mg/kg,6 mg/kg,and 8 mg/kg were administered to three different mice.The LD_(50) was calculated as the geometric mean of the lowest dose killing a mouse and the highest dose showing no death.The invivo /in-vitro antitrypanosomal evaluations were carried out in experimental animals and tissue cell culture respectively. Results:The result of the in vitro studies shows the inhibitive concentration-50(IC-50) against Trypanosoma brucei rhodesiense(T.b.rhodesiense) to be 0.372 mg/kg,while the control drug melarsoprol was 0.006 mg/kg.On Trypanosoma brucei brucei(T.cruzi),the IC-50 is 6.42 mg/kg against 0.87 of the reference drug Benznidazole.The cytotoxicity on L-6 cells exhibited an IC-50 of 0.039 6 mg/kg against the reference d

关 键 词：Zapoteca portoricensis Trypanocidal effects T.b.rhodensiense T.cruzi In vitro/in VIVO model 

分 类 号：R96[医药卫生—药理学]