LipL21 mRNA expression in lungs of hamsters infected with pathogenic Leptospira  

作　　者：Chintana Chirathaworn Namo Suksomyos Somchai Utivamek Somboon Keelawat Duangjai Suwancharoen Duangporn Phulsuksombati Yong Poovorawan 

机构地区：[1]Department of Microbiology,Faculty of Medicine,Chulalongkorn University,Bangkok,Thailand [2]Medical Technology Programme,Faculty of Allied Health Sciences,Chulalongkorn University,Bangkok,Thailand [3]Department of Pathology,Faculty of Medicine,Chulalongkorn University,,Bangkok,Thailand [4]National Institute of Animal Health,Department of Livestock Development,Bangkok,Thailand [5]Department of Biochemistry,Armed Forces Research Institute of Medical Science(Thai Component) [6]Department of Pediatrics,Faculty of Medicine,Chulalongkorn University,Bangkok,Thailand

出　　处：《Asian Pacific Journal of Tropical Medicine》2009年第5期8-13,共6页亚太热带医药杂志（英文版）

基　　金：supported by National Research Council of Thailand and Rachadapiseksompoj Grant, Faculty of Medicine,Chulalongkorn University, Thailand.

摘　　要：Objective:Pulmonary haemorrhage is an increasing cause of death in leptospirosis patients.However,molecular mechanism underlying pathologies in this organ is not clearly understood.It has been shown that sodium transport was disturbed following Leptospira infection.LipL21 is the second abundant outer membrane protein found only in pathogenic Leptospira.Its expression in vivo has been shown which suggests that this protein may be involved in survival in hosts or pathogenesis.However,the expression of this protein in host organs and its role in lung pathology has not been demonstrated.In this study we demonstrated the expression of LipL21 in lungs of hamsters infected with pathogenic Leptospira.Methods:Lung tissues were collected from Golden Syrian hamsters injected with Leptospira interrogans serovar Pyrogenes at days 3,5 and 7 post-infection.Four hamsters were used for each time point.Lungs from non-infected hamsters were collected as a control group.LipL21 mRNA expression in lung tissues was investigated by reverse transcription and nested PCR.Results:LipL21 mRNA expression was detected in all lung tissues from hamsters infected with pathogenic Leptospira.No PCR product was detected when tissues from non-infected hamsters were investigated.Conclusion:Our data demonstrated that LipL21 is expressed in lungs of hamsters infected with pathogenic Leptospira.Additional experiments such as quantitation and localization of LipL21 expression in lungs will provide further information whether this protein is involved in pathogenesis.Objective:Pulmonary haemorrhage is an increasing cause of death in leptospirosis patients.However,molecular mechanism underlying pathologies in this organ is not clearly understood.It has been shown that sodium transport was disturbed following Leptospira infection.LipL21 is the second abundant outer membrane protein found only in pathogenic Leptospira.Its expression in vivo has been shown which suggests that this protein may be involved in survival in hosts or pathogenesis.However,the expression of this protein in host organs and its role in lung pathology has not been demonstrated.In this study we demonstrated the expression of LipL21 in lungs of hamsters infected with pathogenic Leptospira.Methods:Lung tissues were collected from Golden Syrian hamsters injected with Leptospira interrogans serovar Pyrogenes at days 3,5 and 7 post-infection.Four hamsters were used for each time point.Lungs from non-infected hamsters were collected as a control group.LipL21 mRNA expression in lung tissues was investigated by reverse transcription and nested PCR.Results:LipL21 mRNA expression was detected in all lung tissues from hamsters infected with pathogenic Leptospira.No PCR product was detected when tissues from non-infected hamsters were investigated.Conclusion:Our data demonstrated that LipL21 is expressed in lungs of hamsters infected with pathogenic Leptospira.Additional experiments such as quantitation and localization of LipL21 expression in lungs will provide further information whether this protein is involved in pathogenesis.

关 键 词：LEPTOSPIROSIS LEPTOSPIRA LIPL21 Lung HAEMORRHAGE 

分 类 号：R514[医药卫生—内科学]