Molecular characterization,biological forms and sporozoite rate of Anopheles stephensi in southern Iran  

作　　者：Ali Reza Chavshin Mohammad Ali Oshaghi Hasan Vatandoost Ahmad Ali Hanafi-Bojd Ahmad Raeisi Fatemeh Nikpoor 

机构地区：[1]Social Determinants of Health Research Center,Urmia University of Medical Sciences [2]Department of Medical Entomology and Vector Control,School of Public Health,Urmia University of Medical Sciences(UMSU) [3]Department of Medical Entomology and Vector Control,School of Public Health,Tehran University of Medical Sciences(TUMS)

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2014年第1期47-51,共5页亚太热带生物医学杂志（英文版）

基　　金：financially supported by Tehran University of Medical Sciences(TUMS)(Grant No.89-01-27-10448)

摘　　要：Objective:To identify the biological forms,sporozoite rate and molecular characterization of the Anopheles stephensi(An.stephensi)in Hormozgan and Sistan-Baluchistan provinces,the most important malarious areas in Iran.Methods:Wild live An.stephensi samples were collected from different malarious areas in southern Iran.The biological forms were identified based on number of egg-ridges.Molecular characterization of biological forms was verified by analysis of the mitochondrial cytochrome oxidase subunit I and II(mtDNA-COI/COII).The Plasmodium infection was examined in the wild female specimens by species-specific nested-PCR method.Results:Results showed that all three biological forms including mysorensis,intermediate and type are present in the study areas.Molecular investigations revealed no genetic variation between mtDNA COI/COII sequences of the biological forms and no Plasmodium parasites was detected in the collected mosquito samples.Conclusions:Presence of three biological forms with identical sequences showed that the known biological forms belong to a single taxon and the various vectorial capacities reported for these forms are more likely corresponded to other epidemiological factors than to the morphotype of the populations.Lack of malaria parasite infection in An,stephensi,the most important vector of malaria,may be partly due to the success and achievement of ongoing active malaria control program in the region.Objective:To identify the biological forms,sporozoite rate and molecular characterization of the Anopheles stephensi(An.stephensi)in Hormozgan and Sistan-Baluchistan provinces,the most important malarious areas in Iran.Methods:Wild live An.stephensi samples were collected from different malarious areas in southern Iran.The biological forms were identified based on number of egg-ridges.Molecular characterization of biological forms was verified by analysis of the mitochondrial cytochrome oxidase subunit I and II(mtDNA-COI/COII).The Plasmodium infection was examined in the wild female specimens by species-specific nested-PCR method.Results:Results showed that all three biological forms including mysorensis,intermediate and type are present in the study areas.Molecular investigations revealed no genetic variation between mtDNA COI/COII sequences of the biological forms and no Plasmodium parasites was detected in the collected mosquito samples.Conclusions:Presence of three biological forms with identical sequences showed that the known biological forms belong to a single taxon and the various vectorial capacities reported for these forms are more likely corresponded to other epidemiological factors than to the morphotype of the populations.Lack of malaria parasite infection in An,stephensi,the most important vector of malaria,may be partly due to the success and achievement of ongoing active malaria control program in the region.

关 键 词：ANOPHELES STEPHENSI Mysorensis Type Intermediate MTDNA MARKERS Molecular systematic Iran 

分 类 号：Q96[生物学—昆虫学]