Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour  

作　　者：Sourov Kuri Mustahsan Billah S.M.Masud Rana Zannatul Naim Mahmodul Islam Hasanuzzaman Ramjan Ali Rana Banik 

机构地区：[1]Department of Pharmacy,Noakhali Science and Technology University [2]Department of Pharmacy,Atish Dipankar University of Science and Technology

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2014年第4期299-305,共7页亚太热带生物医学杂志（英文版）

基　　金：Supported by the Department of Physiology,Biochemistry and Pharmacology,Faculty of Veterinary Medicine,Chittagong Veterinary and Animal Sciences University,Chittagong,Bangladesh(Grant No.RFLDC/MSC/res.sund/5004/3096)

摘　　要：Objective:To study the phytochemical and biological properties(antioxidant,anthelmintic and thrombolytic)of methanolic extracts of Enhydra fluctuant Lour.,a plant belonging to the Asteraceae family.Methods:The phytochemical evaluation was carried out by qualitative analysis.In vitro antioxidant activity of extract was studied using free radical scavenging assay,ability of reduction,total phenol and total flavonoid contents determination assays.The anthelmintic activity was determined using paralysis and death time of Pheretima posthuma(earthworm)and thrombolytic activity by clot disruption assay.Results:The phytochemical evaluation showed significant presence of flavonoids,triterpenes,carbohydrate,reducing sugars,saponins,phenols,diterpenes,protein and tannin.The antioxidant activity was found significant[IC_(50)(135.20±036)μg/mL]as compared to ascorbic acid[(130.00±0.76)μg/mL].The reducing power was increased with concentration.Total phenol and total flavonoid contents were(153.08±0.38)mg/mL and(172.04±0.56)mg/mL respectively.The paralysis and death time of earthworms for different concentrations of extract were determined and compared with albendazole.The results showed that 10 mg/mL of the crude extract had similar effect with albendazole.Additionally,the crude extract showed a concentration depended relationship with its anthelmintic property.The clot lysis activity of crude extract was compared to the standard streptokinase's clot lysis(40.13%)activity and found significant(31%).Conclusions:The study proves that the crude methanolic extract of Enhydra fluctuant Lour,has significant antioxidant,anthelmintic and thrombolytic activity containing wide range of phytochemicals.Objective:To study the phytochemical and biological properties（antioxidant,anthelmintic and thrombolytic）of methanolic extracts of Enhydra fluctuant Lour.,a plant belonging to the Asteraceae family.Methods:The phytochemical evaluation was carried out by qualitative analysis.In vitro antioxidant activity of extract was studied using free radical scavenging assay,ability of reduction,total phenol and total flavonoid contents determination assays.The anthelmintic activity was determined using paralysis and death time of Pheretima posthuma（earthworm）and thrombolytic activity by clot disruption assay.Results:The phytochemical evaluation showed significant presence of flavonoids,triterpenes,carbohydrate,reducing sugars,saponins,phenols,diterpenes,protein and tannin.The antioxidant activity was found significant[IC50（135.20±036）μg/mL]as compared to ascorbic acid[（130.00±0.76）μg/mL].The reducing power was increased with concentration.Total phenol and total flavonoid contents were（153.08±0.38）mg/mL and（172.04±0.56）mg/mL respectively.The paralysis and death time of earthworms for different concentrations of extract were determined and compared with albendazole.The results showed that 10 mg/mL of the crude extract had similar effect with albendazole.Additionally,the crude extract showed a concentration depended relationship with its anthelmintic property.The clot lysis activity of crude extract was compared to the standard streptokinase’s clot lysis（40.13%）activity and found significant（31%）.Conclusions:The study proves that the crude methanolic extract of Enhydra fluctuant Lour,has significant antioxidant,anthelmintic and thrombolytic activity containing wide range of phytochemicals.

关 键 词：ENHYDRA fluctuans Lour. PHYTOCHEMICALS Antioxidant DPPH radical SCAVENGING PHERETIMA posthuma STREPTOKINASE 

分 类 号：R284[医药卫生—中药学]