A comparative evaluation of methicillin-resistant staphylococci isolated from harness racing-horses,breeding mares and riding-horses in Italy  

作　　者：Karina Mallardo Sandra Nizza Filomena Fiorito Ugo Pagnini Luisa De Martino 

机构地区：[1]Department of Pathology and Animal Health,Infectious Diseases Section,University of Naples "Federico Ⅱ"

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2013年第3期169-173,共5页亚太热带生物医学杂志（英文版）

基　　金：Supported by Faculty of Veterinary Medicine.University of Naples"Federico II".Naples Italy

摘　　要：Objective:To investigate the prevalence of methicillin-resistant staphylococci(MRS)which is a potencial risk factor of transmission between animals and humans in different types of horses(harness racing-horses,breeding mares and riding-horses)and to compare the antimicrobial resistance of the isolates.Methods:A total of 191 healthy horses,housed at different locations of the Campania Region(Italy),were included in the study.Nasal swab samples were collected from each nostril of the horses.The mecA gene was detected by a nested PCR technique.Antibiotic susceptibility was tested for each isolate.Results:MRS was isolated from nasal samples of 68/191(35.6%;95%CI:28.9%-42.9%)healthy horses.All isolates were coagulase-negative with the exception of two coagulase-positive MRS strains,identified as Staphylococcus aureus and Staphylococcus pseudintermedius.2/83(2.4%;95%,CI:0.4%-9.2%).Interestingly,both coagulase-positive MRS isolates were from harness racing-horses.These horses also presented a significantly higher positivity for MRS(53.3%;95%CI:40.1%-66.1%)than the breeding mares and riding-horses groups.Antibiotic susceptibility testing showed difference between isolates due to different origins except for an almost common high resistance to aminopenicillins,such as ampicillin and amoxicillin.Conclusions:It can be concluded that harness racing-horses may act as a significant reservoir of MRS as compared to breeding mares and riding-horses.Objective:To investigate the prevalence of methicillin-resistant staphylococci(MRS)which is a potencial risk factor of transmission between animals and humans in different types of horses(harness racing-horses,breeding mares and riding-horses)and to compare the antimicrobial resistance of the isolates.Methods:A total of 191 healthy horses,housed at different locations of the Campania Region(Italy),were included in the study.Nasal swab samples were collected from each nostril of the horses.The mecA gene was detected by a nested PCR technique.Antibiotic susceptibility was tested for each isolate.Results:MRS was isolated from nasal samples of 68/191(35.6%;95%CI:28.9%-42.9%)healthy horses.All isolates were coagulase-negative with the exception of two coagulase-positive MRS strains,identified as Staphylococcus aureus and Staphylococcus pseudintermedius.2/83(2.4%;95%,CI:0.4%-9.2%).Interestingly,both coagulase-positive MRS isolates were from harness racing-horses.These horses also presented a significantly higher positivity for MRS(53.3%;95%CI:40.1%-66.1%)than the breeding mares and riding-horses groups.Antibiotic susceptibility testing showed difference between isolates due to different origins except for an almost common high resistance to aminopenicillins,such as ampicillin and amoxicillin.Conclusions:It can be concluded that harness racing-horses may act as a significant reservoir of MRS as compared to breeding mares and riding-horses.

关 键 词：METHICILLIN-RESISTANT STAPHYLOCOCCI MARES HARNESS racing-horse Riding-horse Nasal swabs 

分 类 号：S852.6[农业科学—基础兽医学]