Clonal distribution and possible micro evolution of methicillin-resistant Staphylococcus aureus strains in a teaching hospital in Malaysia  

作　　者：Xin Ee Tan Hui-min Neoh Salasawati Hussin Noraziah Mohamad Zin 

机构地区：[1]Department of Biomedical Science,Faculty of Allied Health Sciences [2]UKM Medical Molecular Biology Institute(UMBI) [3]Department of Medical Microbiology and Immunology,Universiti Kebangsaan Malaysia [4]Programme of Biomedical Science,School of Diagnostic and Applied Health Sciences,Faculty of Health Sciences Universiti Kebangsaan Malaysia

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2013年第3期224-228,共5页亚太热带生物医学杂志（英文版）

基　　金：Supported by the Ministry of Higher Education of Malaysia(GrantNo.UKM-NN-03-FRGS 0042-2009);UKM Research University Fund(Grant No.UKMGUP-TKP-08-19-067)

摘　　要：Objective:To genotypically characterize methicillin-resistant Staphylococcus aureus(MRSA)strains isolated from medical and surgical wards in Universiti Kebangsaan Malaysia Medical Centre(UKMMC)in 2009.Methods:MRSA strains were collected and molecularly typed by pulsed-field gel electrophoresis(PFGE).Results:PFGE typing on 180 MRSA isolated in UKMMC identified 5 pulsotypes(A-E)and 6 singletons,where pulsotypes B and C were suspected to he divergent clones originating from a single ancestor.This study also showed that most MRSA strains were isolated from swab(119 isolates),followed by blood(22 isolates),tracheal aspirate(11isolates)and sputum(10 isolates).On the other hand,urine and bone isolates were less,which were 4 and 1 isolates,respectively.The distribution of different pulsotypes of MRSA among wards suggested that MRSA was communicated in surgical and medical wards in UKMMC,with pulsotype B MRSA as the dominant strain.Besides,it was found that most deceased patients were infected by pulsotype B MRSA,however,no particular pulsotype could be associated with patient age,underlying disease,or ward of admittance.Conclusions:Five pulsotypes of MRSA and 6singletons were identified,with pulsotype B MRSA as the endemic strains circulating in these wards,which is useful in establishment of preventive measures against MRSA transmission.Objective:To genotypically characterize methicillin-resistant Staphylococcus aureus(MRSA)strains isolated from medical and surgical wards in Universiti Kebangsaan Malaysia Medical Centre(UKMMC)in 2009.Methods:MRSA strains were collected and molecularly typed by pulsed-field gel electrophoresis(PFGE).Results:PFGE typing on 180 MRSA isolated in UKMMC identified 5 pulsotypes(A-E)and 6 singletons,where pulsotypes B and C were suspected to he divergent clones originating from a single ancestor.This study also showed that most MRSA strains were isolated from swab(119 isolates),followed by blood(22 isolates),tracheal aspirate(11isolates)and sputum(10 isolates).On the other hand,urine and bone isolates were less,which were 4 and 1 isolates,respectively.The distribution of different pulsotypes of MRSA among wards suggested that MRSA was communicated in surgical and medical wards in UKMMC,with pulsotype B MRSA as the dominant strain.Besides,it was found that most deceased patients were infected by pulsotype B MRSA,however,no particular pulsotype could be associated with patient age,underlying disease,or ward of admittance.Conclusions:Five pulsotypes of MRSA and 6singletons were identified,with pulsotype B MRSA as the endemic strains circulating in these wards,which is useful in establishment of preventive measures against MRSA transmission.

关 键 词：Hospital infection MICROEVOLUTION MRSA PFGE TYPING 

分 类 号：R378[医药卫生—病原生物学]