Screening and characterization of extracelluar L-asparaginase producing Bacillus subtilis strain hswx88,isolated from Taptapani hotspring of odisha,India  

作　　者：Biswaprakash Pradhan Sashi K Dash Sabuj Sahoo 

机构地区：[1]Pharmaceutical Biotechnology Division,University Department of Pharmaceutical Sciences,Utkal University [2]Department of Seed Technology,Orissa University of Agriculture and Technology

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2013年第12期936-941,共6页亚太热带生物医学杂志（英文版）

基　　金：Support by the All India Council for Technical Education.New Delhi,India through"Research Promotion Scheme"(AICTE Letter-F.No.8023/RID/RPS-17/(POLICYIV)/(GOVT.)/2011-12.Dt:09/07/2012,Veh.No.:340)

摘　　要：Objective:To screen and isolate an eco-friendly,u thermophilic and potent L-asparaginase producing bacterium,with novel immunological properties that may obviates hypersensitivity reactions.Methods:In the present study baclerial strain isolated for extracellular L-asparaginase production from hotspring,identified by morphological,biochemical and physiological tests followed by t6S rDNA technology and the L-asparaginase production ability was tested by both semi quantitative and quantitative enzymatic assay.Result:The bacterial strain was identified as Bacillus sublilis strain hswx88(GenBank Accession Number JQ237656.1).The extracellular enzyme yielding capacity isolate Bacillus subtilis strain hswx88(23.8 IU/mL)was found to be 1.7 and 14.5 limes higher than the reference organism Pectobacterium carotovorum MTCC 1428(14.2 IU/mL)and Bacillus sp.BCCS 034(1.64 IU/mL).Conclusion:The isolate is eco-friendly and useful to produce bulk quantity of extracellular,thermophilic L-asparaginase for the treatment of various tumor cases and for preparation of acrylamide free fry food preparation.Objective:To screen and isolate an eco-friendly,u thermophilic and potent L-asparaginase producing bacterium,with novel immunological properties that may obviates hypersensitivity reactions.Methods:In the present study baclerial strain isolated for extracellular L-asparaginase production from hotspring,identified by morphological,biochemical and physiological tests followed by t6S rDNA technology and the L-asparaginase production ability was tested by both semi quantitative and quantitative enzymatic assay.Result:The bacterial strain was identified as Bacillus sublilis strain hswx88(GenBank Accession Number JQ237656.1).The extracellular enzyme yielding capacity isolate Bacillus subtilis strain hswx88(23.8 IU/mL)was found to be 1.7 and 14.5 limes higher than the reference organism Pectobacterium carotovorum MTCC 1428(14.2 IU/mL)and Bacillus sp.BCCS 034(1.64 IU/mL).Conclusion:The isolate is eco-friendly and useful to produce bulk quantity of extracellular,thermophilic L-asparaginase for the treatment of various tumor cases and for preparation of acrylamide free fry food preparation.

关 键 词：L-ASPARAGINASE THERMOPHILIC bacteria BACILLUS SUBTILIS STRAIN hswx88 16S rDNA 

分 类 号：TQ460.1[化学工程—制药化工] R94[医药卫生—药剂学]