Serological investigation of vector-borne disease in dogs from rural areas of China  

作　　者：Shiwen Wang Jing He Lijuan Zhang 

机构地区：[1]National Institute of Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention [2]Department of Infectious Diseases,302 Hospital of PLA

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2012年第2期102-103,共2页亚太热带生物医学杂志（英文版）

基　　金：supported by the National Basic Research Program of China(973 Program)2010CB530200(2010CB530206);the China-US Collaborative Program on Emerging and Re-emerging Infectious Disease(No.1U2GGH000018-01)

摘　　要：Objective:To evaluate the Anaplasma phagocytophilum(A.phagocytophilum),Ehrlichia canis(E.canis,Dirofilaria immitis(D.immitis)(canine heartworm),Borrelia burgdorferi(B.burgdorferi)infections in countryside dogs from Yunnan,Hainan and Anhui provinces.Methods:Serum samples were collected from 26 dogs in Yunnan.Hainan and Anhui provinces.The samples were tested using a commercial ELISA rapid diagnostic assay kit(SNAP^(?)4Dx^(?);IDEXX Laboratories,Inc.U.S.A.).Meaiiwliile,indirect immunofluorescence assay(IFA)recommended by WHO was conducted to delect IgG to A.phagocytophilum.Two methods were analyzed and compared.Results:The number of serologically positive dogs for IgG to A.phagocytophilum was only 2which was from Hainan province and none of the 26 dogs responded positive for E.canu.D.immitis(canine heartworm,and B.burgdorferi by ELISA rapid diagnostic method.The number of serologically positive dogs for IgG to A.phagocytophilum was 13(50%)by IFA method.Data of the two methods were analyzed by statistical software and the difference was statistically significant(P=0.002).Conclusions:It can be concluded that IFA method was more sensitive than ELISA rapid diagnostic method.However,we need conduct further and intensive epidemiology survey on tick-born diseases pathogens including.4.phagocytophilum,E.canis,D.immitis(canine heartworm),and B.burgdorferi which have public health significance.Objective:To evaluate the Anaplasma phagocytophilum(A.phagocytophilum),Ehrlichia canis(E.cams),Dirofilaria immitis(D.immitis)(canine heartworm),Borrelia burgdorferi(B.burgdorferi)infections in countryside flogs from Yunnan.Hainan and Anhui provinces.Methods:Serum samples were collected from 26 dogs in Yunnan,Hainan and Anhui provinces.The samples were tested using a commercial ELISA rapid diagnostic assay kit(SNAP(?) 4Dx(?):IDEXX Laboratories,Inc.U.S.A.).Meanwhile,indirect immunofluorescence assay(IFA) recommended by WHO was conducted to detect IgG to A.phagocytophilum.Two methods were analyzed and compared.Results:The number of serologically positive dogs for IgG to.4.phagocytophilum was only 2which was from Hainan province and none of the 26 dogs responded positive for E.ranis.D.immitis(canine heartworm).and B.burgdorferi by ELISA rapid diagnostic method.The number of serologically positive dogs for IgG to A.phagocytophilum was 13(50%by IFA melhod.Data of the two methods were analyzed by statistical software and the difference was statistically significant(P=0.002).Conclusions:It can be concluded that IFA method was more sensitive than ELISA rapid diagnostic melhod.However,we need conduct further and intensive epidemiology survey on tick-born diseases pathogens including A.phagocytophilum,E.canis.D.immitis(canine heartworm).and B.burgdorferi which have public health significance.

关 键 词：ANAPLASMA phagocytophilum EHRLICHIA canis BORRELIA BURGDORFERI DIROFILARIA immitis DOGS China Vector-borne disease Serological investigation ELISA rapid diagnostic assay 

分 类 号：R181.3[医药卫生—流行病学]