Effect of germicidal UV-C light(254 nm)on eggs and adult of house dustmites,Dermatophagoides pteronyssinus and Dermatophagoides farinae(Astigmata:Pyroglyhidae)  

作　　者：Ernieenor Faraliana Che Lah Raja Noor Azreen Raja Musa Ho Tze Ming 

机构地区：[1]Acarology Unit,Infectious Disease Research Centre,Institute for Medical Research

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2012年第9期679-683,共5页亚太热带生物医学杂志（英文版）

摘　　要：Objective:To examined the immediate and 24 hours post-irradiation germicidal effects of UV-C lamp on.eggs and adults of house dust mites Dermatophagoides pteronyssinus(D.pteronyssinus)and Dermatophagoides farinae(D.farinae).Methods:This study investigated the immediate and24 hours post irradiation mortalities of adult mites exposed to UV-C at different exposure times(5mins,10 mins,15 mins,20 mins,30 mins and 60 mins)and distances(10 cm,25 cm,33 cm,45 cm and 55 cm).Fresh eggs of the 2 dust mites were also irradiated at 10,35 and 55 cm for 0.5,1,2,3,and 5 minutes,and observed daily post-irradiation for up to 7 days.Results:Highest immediate mortality of 100%occurred with direct irradiation al 10 cm distance from UV-C lamp and for 60mins,for both species of mites.The post 24 hours mean mortality rates were(58.4±17.4)%for D.pteronyssinus and(27.7±9.7)%for D.fitrinae when irradiated for 1 hour at 55 cm distance under UV-C lamp.When mites were irradiated in the presence of culture media,the highest mortality rates were lower compared to the direct irradiation;at 10 cm distance and 60 mins exposure,the mean mortality was(74.0±6.8)%for D.pteronyssinus and(70.3±6.7)%for D.farinae.Egg hatchability for both species of mites was also notably reduced by greater than 50%following irradiation.Conclusions:Ultraviolet C irradiation is lethal to an array of organisms by damaging their nucleic acids(DNA and RNA).This study demonstrates the increasing mite mortalities with increasing exposure times and decreasing distances.Objective:To examined the immediate and 24 hours post-irradiation germicidal effects of UV-C lamp on eggs and adults of house dust mites Dermatophagoides pteronyssinus(D.pteronyssinus) and Dermatophagoides farinae(D.farinae).Methods:This study investigated the immediate and 24 hours post irradiation mortalities of adult mites exposed to UV-C at different exposure times(5 mins,10 mins,15 mins,20 mins,30 mins and 60 mins) and distances(10 cm,25 cm,35 cm,45 cm and 55 cm).Fresh eggs of the 2 dust mites were also irradiated at 10,35 and 55 cm for 0.5,1,2,3, and 5 minutes,and observed daily post-irradiation for up to 7 days.Results:Highest immediate mortality of 100%occurred with direct irradiation at 10 cm distance from UV-C lamp and for 60 mins,for both species of mites.The post 24 hours mean mortality rates were(58.4±17.4)%for D. pteronyssinus and(27.7±9.7)%for D.farinae when irradiated for 1 hour at 55 cm distance under UV-C lamp.When mites were irradiated in the presence of culture media,the highest mortality rates were lower compared to the direct irradiation;at 10 cm distance and 60 mins exposure, the mean mortality was(74.0±6.8)%for D.pteronyssinus and(70.3±6.7)%for D.farinae.Egg hatchability for both species of mites was also notably reduced by greater than 50%following irradiation.Conclusions:Ultraviolet C irradiation is lethal to an array of organisms by damaging their nucleic acids(DNA and RNA).This study demonstrates the increasing mite mortalities with increasing exposure times and decreasing distances.

关 键 词：Physical control UV-IRRADIATION DERMATOPHAGOIDES farinae DERMATOPHAGOIDES pteronyssinus MORTALITY 

分 类 号：R384[医药卫生—医学寄生虫学]