机构地区:[1]Entomology Research Institute,Loyola College [2]PG and Research Department of Advanced Zoology and Biotechnology,Loyola College
出 处:《Asian Pacific Journal of Tropical Biomedicine》2012年第12期987-991,共5页亚太热带生物医学杂志(英文版)
基 金:Supported by Department of Science and Technology,New Delhi(Grant No.SR/SO/AS-03/2004)
摘 要:Objective:To evaluate the efficacy of Atalantia monophylla(A.monophylla)leaf in different solvent crude extracts and fractions against eggs of Spodoptera litura(S.litura).Methods:Hexane,ethyl acetate and chloroform solvent extracts of A.monophylla leaf and 12 fractions from hexane extract were screened at 5.0%t,2.5%,1.0%and 0.5%for crude extracts and 1000,500,250 and 125 mg/kg for fractions against the eggs of S.litura for the ovicidal activity.LC_(50)and LC_(90)were calculated using probit analysis.Results:Hexane crude extract showed maximum ovicidal activity of 61.94%at 5.0%concentration with a correlation value of r^2=0.81,and least LC_(50)value of3.06%.Hexane extract was fractionated using silica gel column chromatography and 12 fractions were obtained.Fraction 9 was active which showed maximum ovicidal activity of 75.61%at 1000mg/kg with the LC_(50)value of 318.65 mg/kg and LC_(90)value of 1473.31 mg/kg.In linear regression analysis,significant and high correlation(r^2=0.81%)was seen between concentration and ovicidal activity of hexane crude extracts and its active frsctien.Conclusions:As per our knowledge,this is the first report for ovicidal activity of A.monophylla against S.litura,A.monophylla could be used for the management of S.litura and other insect pests.Objective:To evaluate the efficacy of Atalantia monophylla(A.monophylla) leaf in different solvent crude extracts and fractions against eggs of Spodoptera lilura(S.litura).Methods: Hexane,ethyl acetate and chloroform solvent extracts of A.monophylla leaf and 12 fractions from hexane extract were screened at 5.0%,2.5%,t.0%and 0.5%for crude extracts and 1000,500, 250 and 125 mg/kg for fractions against the eggs of S.litura for the ovicidal activity.LC_(50) and LC_(60) were calculated using probit analysis.Results:Hexane crude extract showed maximum ovicidal activity of 61.94%at 5.0%,concentration with a correlation value of r^2=0.81,and least LC_(50) value of 3.06%.Hexane extract was fractionated using silica gel column chromatography and 12 fractions were obtained.Fraction 9 was active which showed maximum ovicidal activity of 75.61%at 1000 mg/kg with the LC_(50),value of 318.65 mg/kg and LC_(90) value of 1473.31 mg/kg.In linear regression analysis,significant and high correlation(r^2=0.81%) was seen between concentration and ovicidal activity of hexane crude extracts and its active fraction.Conclusions:As per our knowledge,this is the first report for ovicidal activity of A.monophytta against S.litwa,A.monophylla could be used for the management of S.litura and other insect pests.
关 键 词:Atalantia monophylla CRUDE extracts FRACTIONS OVICIDAL activity SPODOPTERA litura
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