来源于解淀粉芽胞杆菌的芸薹根肿菌抑菌活性物质的分离与鉴定  

Separation and Characterization of Bacillus amyloliquefaciens Active Substances Against Plasmodiophora brassicae

作　　者：刘晓艳[1] 张薇[2] 闵勇[1] 张亚妮[1] 张志刚[1] 江爱兵[1] 杨自文[1] 

机构地区：[1]湖北省农业科学院湖北省生物农药工程研究中心,湖北武汉430064 [2]湖北生物科技职业学院园艺园林学院,湖北武汉430070

出　　处：《氨基酸和生物资源》2014年第1期39-43,共5页Amino Acids & Biotic Resources

基　　金：国家科技支撑计划(2011BAB06B004_02);国家863科技计划(2011AA10A201)

摘　　要：十字花科根肿病是由芸薹根肿菌引起的较为严重的世界性病害之一,至今还无有效控制该病害的生物农药。本研究通过盆栽实验,筛选到一株解淀粉芽胞杆菌HB_26,对根肿病原菌具有60%以上的抑制活性。通过高效液相质谱(LC_MS)对活性物质进行分离纯化,根据分子量和紫外吸收光谱初步鉴定活性物质为肽类,命名为BA30。抑真菌实验证明BA30在150μg·mL-1的浓度下,对小麦赤霉和灰霉病菌有70%的抑菌活性,对蚕豆锈病和水稻纹枯病菌有50%的抑菌活性,对番茄早疫病菌有30%的抑菌活性。Clubroot which is caused by Plasmodiophora brassicae,is a serious disease of brassica crops throughout the world. No any effective ways of controlling this pathogen is now available. B. amyloliquefaciens strain HB-26 with more than 60% inhibition activity against P. brassicae by Chinese cabbage pot experiment was studied. The active substance namely BA30 was separated and purified by HPLC,and it was identified to be a peptide derivative by mass spectrometry. In vitro inhibition test showed that BA30 had mutiple antifungal activity:inhibitory rate of 70% against Fusarium graminearum and Botrytis cinerea,50% against Uromyces fabae and Thanatephorus cucumeris,30% against Alternaria solani at the concentration of 150 μg·mL-1. Based on these results,we suggest that HB-26 or BA30 has potential application for the control of clubroot of brassica crops.

关 键 词：十字花科根肿病 解淀粉芽胞杆菌 芸薹根肿菌 抗菌肽 分离与鉴定 

分 类 号：Q939.1[生物学—微生物学]