慢病毒介导shRNA沉默人纤维介素基因对心肌微血管内皮细胞增殖、迁移的影响  

Effect of shRNA silencing fgl2 gene induced by lentivirus on microvascular endothelial cells of myocardial proliferation and migration

作　　者：王亮[1] 吴友平[1] 郑振中[1] 殷然[1] 王梦洪[1] 郑泽琪[1] 彭景添[1] 魏云锋[1] 

机构地区：[1]南昌大学第一附属医院心血管内科,江西南昌330006

出　　处：《中国老年学杂志》2014年第11期3030-3032,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金(No.30570728);江西省卫生厅科研项目(No.20081022)

摘　　要：目的探讨人纤维介素(fgl2)对心肌微血管内皮细胞(MMVECs)增殖、迁移的影响。方法制作并包装fgl2的RNA干扰慢病毒,转染MMVECs。实验分为单纯MMVECs(对照组)、空载质粒慢病毒(GFP组)、fgl2短发夹RNA(shRNA)慢病毒(fgl2-RNAi-LV组)。稳定转染4 d后,实时定量RT-PCR(qRT-PCR)检测各组fgl2 mRNA表达,MTT检测细胞增殖;Transwell检测细胞迁移能力。结果 qRT-PCR结果显示,与对照组与GFP组比较,fgl2-RNAi-LV组的fgl2表达明显下调,MMVECs的增殖能力与迁移能力增强(P<0.05),而对照组与GFP组无明显差异。结论转染fgl2shRNA慢病毒载体后MMVECs的fgl2表达水平明显下调,fgl2shRNA慢病毒转染MMVECs,MMVECs的增殖能力与迁移能力增强。Objective To construct RNAi lentivirus vector of fgl 2 gene and explore the effect of fgl 2 gene on microvascular endothe-lial cells ( MMVECs) of myocardial proliferation and migration.Methods RNAi lentivirus vector of fgl 2 gene was constructed and infected into MMVECs.Cells were divided into pure MMVECs ( control group ) , empty plasmid lentivirus ( GFP group ) , fgl2 short hairpin RNA (shRNA group), lentivirus (fgl2-RNAi-LV group).4 days later transfected into cells, quantitative real-time RT-PCR (Qrt-PCR) was used to detect fgl2 mRNA expression , MTT was used to detect cell proliferation , Transwell was used to detect cell migration.Results Compared with control and GFP groups , fgl2 expression was down-regulated and proliferation and migration of MMVECs were increased in fgl 2-RNAi-LV group ( P<0.05 ).There were no significant differences in above indexes between control and GFP groups .Conclusions fgl2 expression in MMVECs transfected with fgl 2-RNAi-LV is down-regulated and proliferation and migration of MMVECs are increased.

关 键 词：纤维介素2(fgl2) 短发夹RNA 心肌微血管内皮(MMVECs) 增殖 迁移 

分 类 号：R33[医药卫生—人体生理学]