天麻素预处理对短暂性脑缺血大鼠脑组织S100β表达的影响  

Effection of Gastrodin on the expression of S100β in rat transient focal cerebral ischemia

作　　者：赵朝华[1] 吴树强[2] 杨杰[3] 苟兴春[1] 张军锋[1] 杨吉平[1] 史利利[1] 

机构地区：[1]西安医学院基础医学研究所,陕西西安710021 [2]西安医学院附属医院胸心外科 [3]西安交通大学医学院人体解剖学系

出　　处：《中国老年学杂志》2014年第8期2156-2158,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金(No.81071060);陕西省教育厅专项科研计划项目(No.2013JK0756);西安医学院科研基金资助项目(10FC012)

摘　　要：目的：探讨天麻素对S100β表达的影响，明确天麻素的神经保护机制。方法采用线栓法阻断大脑中动脉（MCAO）2 h诱导建立暂时性局部脑缺血模型，术前连续3 d给予 SD 大鼠腹腔注射天麻素，免疫组织化染色观察缺血边缘区 S100β阳性细胞数目变化；ELISA 检测血清S100β的浓度。结果天麻素预处理组大鼠S100β阳性细胞数目增加较少，1、3 d与缺血组和生理盐水组比较均有显著差异（ P＜0.05）。 ELISA测定结果显示MCAO后血清S100β含量1 d时迅速升高，3～7 d成倍数下降。天麻素处理组1、3 d大鼠血清S100β浓度明显降低（P＜0.05）。结论天麻素能明显抑制脑缺血再灌注后S100β的过度表达，减轻神经细胞损伤。Objective To explore the effect of Gastrodin on expression of S100β after cerebral post-ischemic reperfusion. Methods Gastrodin was intraperitoneally injected daily for 3days before inducing focal cerebral ischemia in SD rats by intraluminal occlusion of middle cerebral artery( MCAO) for 2 hours. Immunohistchemical method was used to observe the expression of S100β. The concentration of S100β in serum was detected by double-antibodies-sandwich-ELISA method as well. Results After MCAO,the number of S100β positive cells in gastrodin group was significantly decreased compared with the ischemia group at 1 day and 3 day respectively( P<0. 05). The concentrations of S100β in serum remarkablely decreased campared with the ischemia group at 1 and 3 days( P < 0. 05) in Gastrodin group. Conclusions Gastrodin could inhibit the over-expression of S100β,which may be one of the neuroprotective mechanism of Gastrodin.

关 键 词：天麻素 脑缺血 S100Β 

分 类 号：R32[医药卫生—人体解剖和组织胚胎学]