丝胶对糖尿病大鼠胰岛细胞神经肽Y表达的影响  

Effects of sericine on neuropeptide Y expression in islet cells of type 2 diabetes mellitus rats

作　　者：刘平[1] 刘东慧[1] 付秀美[1] 陈志宏[1] 

机构地区：[1]承德医学院,河北承德067000

出　　处：《中国老年学杂志》2014年第8期2182-2184,共3页Chinese Journal of Gerontology

基　　金：河北省自然基金项目(H2012406018);河北省教育厅项目(2006301);河北省科技厅项目(08276101D-19)

摘　　要：目的观察丝胶对2型糖尿病大鼠胰岛细胞神经肽Y(NPY)表达的影响。方法 36只雄性SD大鼠随机分为正常对照组、糖尿病模型组和丝胶治疗组,每组12只。链脲佐菌素腹腔注射建立2型糖尿病大鼠模型并给予丝胶(2.4 g/kg)灌胃。SP免疫组织化学染色法观察大鼠胰岛细胞NPY的表达。结果 NPY蛋白阳性产物位于胰岛α细胞细胞质,呈棕黄色颗粒状。糖尿病模型组大鼠胰岛α细胞NPY蛋白的表达明显高于正常对照组大鼠(P<0.01);丝胶治疗组大鼠胰岛α细胞NPY蛋白的表达明显低于糖尿病模型组大鼠(P<0.01)。结论丝胶可通过降低神经肽Y的表达保护糖尿病时胰岛细胞损伤。Objective To observe the effects of sericine on neuropeptide Y ( NPY) expression in islet cells of type 2 diabetes melli-tus rats.Methods Methods 36 male SD rats were randomly divided into 3 groups with 12 rats in each group:normal control , diabetes mel-litus model and sericine treatment groups .Type 2 diabetes mellitus rat model was made by continuous intraperitoneal injection of streptozoto -cin, and then the rats were lavaged with sericine (2.4g/kg).SP immunohistochemical stain was used to observe NPY expression in islet cells.Results NPY positive immunoreactive products were located in cytoplasm of islet alpha cells .Compared with normal control rats , the NPY expression in alpha cells of rats in diabetes mellitus model group obviously increased (P<0.01).The NPY expression in alpha cells of rats in sericine treatment group was obviously lower than that of rats in diabetes mellitus model group (P<0.01).Conclusions Sericine can protect islet alpha cells by down regulating NPY expression during diabetes mellitus .

关 键 词：丝胶 2型糖尿病 胰岛 神经肽Y 

分 类 号：R587[医药卫生—内分泌]