A METHOD FOR ESTABLISHING AN ANIMAL MODEL OF LIKE-AUDITARY NEUROPATHY  

作　　者：GUO Weiwei CHEN Peipei WEI Xiaoquan YANG Shiming 

机构地区：[1]Department of Otolaryngology-Head and Neck Surgery, Institute of Oto-Neurobiology Centre, Otolaryngology Chinese PLA General Hospital [2]Department of Otolaryngology-Head and Neck Surgery Nanjing Children's Hospital ,Nanjing Medical University

出　　处：《Journal of Otology》2014年第1期48-51,共4页中华耳科学杂志（英文版）

基　　金：supported by grants from the National Basic Research Program of China(973Program);And the National Natural Science Foundation of China(NSFC)(#81271082)

摘　　要：Objective: To establish an animal model of like-auditory neuropathy in neonatal rat. Methods The ani-mals were injected with phenylhydrazine hydrochloride or saline at 7-day of age. ABR and DPOAE were performed to assess the auditory function. The cochlea basilar membrane stretched preparation and cochlear frozen sections were prepared for immunohistochemical staining to examine the morphological change of hair cells and spiral ganglion cells (SGNs). Results At 7-day age the ABR waveI, III, V, latencies andI-III,I-V IWIs in the experimental group were significantly prolonged compared with those in the control group. The ABR thresholds were also elevated in the experimental group. We found there is no significant differ-ence in DPOAE in phenylhydrazine hydrochloride exposure group compare to control group. The cochlear hair cells showed no signs of loss in both group, but the total number of neurofilaments positive cells in SGNs were significantly reduced in the phenylhydrazine treated animals. Conclusion Our study suggests that phenylhydrazine hydrochloride can change the auditory function and induce peripheral nerve pathology by targeted mainly the SGNs in neonatal rat.Objective: To establish an animal model of like-auditory neuropathy in neonatal rat. Methods The ani-mals were injected with phenylhydrazine hydrochloride or saline at 7-day of age. ABR and DPOAE were performed to assess the auditory function. The cochlea basilar membrane stretched preparation and cochlear frozen sections were prepared for immunohistochemical staining to examine the morphological change of hair cells and spiral ganglion cells (SGNs). Results At 7-day age the ABR waveI, III, V, latencies andI-III,I-V IWIs in the experimental group were significantly prolonged compared with those in the control group. The ABR thresholds were also elevated in the experimental group. We found there is no significant differ-ence in DPOAE in phenylhydrazine hydrochloride exposure group compare to control group. The cochlear hair cells showed no signs of loss in both group, but the total number of neurofilaments positive cells in SGNs were significantly reduced in the phenylhydrazine treated animals. Conclusion Our study suggests that phenylhydrazine hydrochloride can change the auditory function and induce peripheral nerve pathology by targeted mainly the SGNs in neonatal rat.

关 键 词：Animal model Auditory brainstem response Distortion-product otoacoustic emission auditory neuropathy 

分 类 号：R764[医药卫生—耳鼻咽喉科]