PFT-α对隐睾所致生精细胞凋亡的影响  

Effect of PFT-α on apoptosis of spermatogenic cells caused by enorchia

作　　者：谢丽 贺丽萍[2] 杨挚鹰[2] 石金凤[3] 龙治峰[3] 谢远杰[3] 

机构地区：[1]长沙卫生职业学院医学基础部,长沙410100 [2]湖南师范大学医学院组织胚胎学教研室,长沙410013 [3]南华大学医学院组织胚胎学教研室,湖南衡阳421001

出　　处：《中南大学学报（医学版）》2014年第3期276-281,共6页Journal of Central South University ：Medical Science

基　　金：湖南省科技厅资助项目(2009FJ3117)~~

摘　　要：目的:通过研究PFT-α对隐睾生精细胞中p53和bcl-2/bax表达的影响,探讨生精细胞凋亡的分子机制。方法:将雄性SD大鼠随机分为假手术组、隐睾组、隐睾+p53抑制剂(p-i t y three inhibitor-alpha,PFT-α)组、隐睾+PFT-α溶媒(DMSO)组。后三组建立单侧隐睾模型,后两组分别于大鼠腹腔内注射PFT-α和PFT-α溶媒,每天1次,定时定量。7 d后处死所有大鼠,取双侧睾丸称湿重。观察手术侧睾丸生精细胞组织形态,采用TUNEL法结合流式细胞术(flow cytometry,FCM)检测生精细胞凋亡程度,免疫组织化学和Western印迹分别检测p53和bcl-2/bax的表达。结果:隐睾+PFT-α组与隐睾组和隐睾+PFT-α溶媒组比较,生精上皮排列有序,细胞层数厚,凋亡指数较低,p53和bax表达弱,bcl-2表达强。结论:PFT-α可能通过上调bcl-2,下调p53和bax的表达,抑制隐睾生精细胞的过度凋亡。Objective: To determine the molecular mechanism of germ cell apoptosis via investigating the effect of PFT-α on the expression of p53 and bcl-2/bax during experimental cryptorchid cell apoptosis. Methods: Male Sprague-Dawley rats were assigned into 4 groups: a sham-operated group, a cryptorchid group, a cryptorchid+p53 inhibitor(p53 inhibitor-alpha, PFT-α) group, and a cryptorchid+dissolvent of PFT-α [dimethyl sulphoxide(DMSO)] group. Unilateral cryptorchidism was surgically induced in the rats of the cryptorchid group, PFT-α group, andcryptorchid+dissolvent of PFT-α group. h e rats in the PFT-α group and cryptorchid+dissolvent of PFT-α group were intra-peritoneally injected PFT-α and dissolvent of PFT-α, respectively, once a day. The rats were killed on the 7th day after the surgery. The morphology of spermatogenic epithelium at the side of surgery in the rats was observed under light microscope. h e apoptosis of spermatogenic cells in the unilateral cryptorchidism was evaluated by TUNEL and l ow cytometry(FCM). The protein expression levels of p53, bcl-2, and Bax were detected by Western blot and immunohistochemical assay in turn. Results: Compared with the cryptorchid groups and the cryptorchid+dissolvent of PFT-α group, the seminiferous epithelium of the cryptorchid+p53 inhibitor group appeared orderly, with thicker cell layers and lower apoptosis index, weak protein expression level of p53/Bax and strong protein expression level of bcl-2. Conclusion: PFT-α inhibits the germ cell apoptosis caused by the experimental cryptorchidism via increasing the expression of bcl-2 and decreasing the expression of p53 and bax.

关 键 词：PFT-Α 生精细胞 凋亡 P53 BCL-2/BAX 

分 类 号：R697[医药卫生—泌尿科学]