骨髓间充质干细胞经肾动脉移植修复小鼠输尿管损伤的方法学研究  

Methodology of BMSCs in repairing ureteral injury in mice via renal artery transplantation

作　　者：李东辉[1] 白志明[1] 李金东[1] 程庆[1] 郭子义[2] 邓湘东[3] 曹卉[3] 

机构地区：[1]中南大学湘雅医学院附属海口医院泌尿外科,海口570208 [2]中南大学湘雅医学院附属海口医院放射科,海口570208 [3]中南大学湘雅医学院附属海口医院中心实验室,海口570208

出　　处：《中南大学学报（医学版）》2014年第3期282-288,共8页Journal of Central South University ：Medical Science

基　　金：海南省自然科学基金(811151)~~

摘　　要：目的:从方法学上研究骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)经肾动脉移植对小鼠输尿管损伤的修复作用,探讨经肾动脉移植BMSCs的有效性.方法:显微血管夹夹毕49只3～4周龄Balb/c小鼠的左侧输尿管,10 d后取出显微血管夹以解除左侧输尿管梗阻,然后将这些实验小鼠随机分为实验组(n=25)及对照组(n=24).实验组小鼠取出显微血管夹后立即经肾动脉移植荧光素酶标记的Balb/c小鼠BMSCs,对照组小鼠取出显微血管夹后随即关闭切口,不移植BMSCs.磁共振成像对实验小鼠进行扫描,通过测量不同时间点的实验小鼠的左肾盂容积,比较实验组与对照组小鼠左侧输尿管梗阻解除后的左输尿管复通率,评估BMSCs对输尿管损伤的修复作用.结果:实验小鼠左侧输尿管梗阻后的左肾盂容积明显大于梗阻前(P＜0.01);实验组与对照组小鼠左侧输尿管梗阻后10d的左肾盂容积差异无统计意义(P=0.693);实验组小鼠左侧输尿管梗阻解除后的左输尿管复通率较对照组小鼠明显增高(P=0.012).结论:BMSCs经肾动脉移植可促进小鼠输尿管损伤的修复.Objective: To investigate the ef ect of bone marrow mesenchymal stem cells(BMSCs) on ureteral injury repair via renal artery transplantation. Methods: h e let ureteral obstruction model was set up in 49 Balb/c mice by micro vascular clamp. h e microscopic vascular clamp was taken out to lit the let ureteral obstruction at er 10 days. h e mice were randomly divided into an experimental group(n=25) and a control group(n=24). Balb/c mice BMSCstransfected by luciferase(Luc) were transplantated immediately through the renal artery at er removing the microscopic vascular clamp from the experimental group; while mice in the control group was closed the incision at er the microscopic vascular clamp was removed immediately and without BMSCs transplant. Magnetic resonance imaging(MRI) was used to scan the experimental mice. By measuring the left renal pelvis volume of the experimental mice at different time points and comparing the left ureter recanalization rate at er removing let ureteral obstruction of the experimental group and the control group, we evaluated the repair ef ect of BMSCs on ureteral injury. Results: h e volume of the let renal pelvis in experimental mice became bigger obviously at er the let ureter was obstructed(P<0.01). h e let renal pelvis volume of the experimental group and the control group had no statistical signii cance 10 days at er the let ureteral obstruction was set up(P=0.693). In the experimental group, the let ureter recanalization rate was higher than that in the control group, at er removing the let ureteral obstruction(P=0.012). Conclusion: Transplantation through the renal artery can promote the restoration of ureteral injury in mice.

关 键 词：骨髓间充质干细胞 单侧输尿管梗阻 经肾动脉移植 磁共振成像 荧光素酶 

分 类 号：R693[医药卫生—泌尿科学]