神经营养素-3预治疗对鼠重复鞘内注入罗哌卡因毒性的影响  

Protective effect of pretreatment with neurotrophin-3 on intrathecal ropivacaine in rats

作　　者：孙志华[1] 郭曲练[1] 许小萍[1] 张重[1] 王娜[1] 宋宗斌[1] 

机构地区：[1]中南大学湘雅医院麻醉科,长沙410008

出　　处：《中南大学学报（医学版）》2014年第1期17-22,共6页Journal of Central South University ：Medical Science

摘　　要：目的:探讨神经营养素-3(NT-3)预治疗对鼠重复鞘内注入罗哌卡因毒性的影响。方法:144只SD雄性大鼠鞘内置管成功后随机分成4组,每组36只。分别给予鞘内注射生理盐水,0.5%罗哌卡因,1%罗哌卡因以及1%罗哌卡因+NT-3各12 h,每次间隔1.5 h。观察1,3,5,7,14,28 d时大鼠行为学,运动功能,机械痛阈(PWMT)和热痛阈(PWTL),病理组织学损伤评分的改变。结果:与生理盐水组和0.5%罗哌卡因组比较,1%罗哌卡因组1,3,5 d PWMT和PWTL显著增高,病理组织学损伤评分1,3,5,7,14 d显著增高(P<0.05);与1%罗哌卡因+NT-3组比较,1%罗哌卡因组1,3,5 d PWMT和PWTL显著增高,病理组织学损伤评分5,7,14 d显著增高(P<0.05)。结论:NT-3预治疗对鼠重复鞘内注入1%罗哌卡因脊髓神经毒性具有明显保护作用。Objective:To investigate the effect of pretreatment with neurotrophin-3(NT-3) on intrathecal ropivacaine in rats.Methods:A total of 144 male Sprague Dawley rats weighing 280–320 g were successfully implanted with microspinal cather following the improved methods of Yaksh.The rats were randomly divided into 4 groups and given saline(Group NS,n=36),0.5% ropivacaine(Group M,n=36),1% ropivacaine(Group R,n=36),and ropivacaine+NT-3(Group T,n=36).The rats received 0.12 mL/kg body weight of ropivacaine at 0.5% or 1%,or normal saline only,via an implanted intrathecal catheter at 90-min interval for 12 h in Group NS,M,R and T.In the meantime the rats also received NT-3 0.1 mg/kg in group T.On days 1,3,5,7,14 and 28,we assessed the paw withdrawal mechanical threshold(PWMT) and paw withdrawal thermal latency(PWTL),behavioural change and histopathological damage score changed for possible neuronal injury within the spinal cord.Results:Compared with Group NS and Group M,the PWMT and PWTL were significantly higher on 1,3,5 d and the histopathological damage score was significantly higher on 1,3,5,7,14 d in Group R(P<0.05).Compared with Group T,the PWMT and PWTL in Group R were significantly higher on 1,3,5 d and histopathological damage score was significantly higher on 5,7,14 d(P<0.05).Conclusion:NT-3 pretreatment in mice has obvious protective effect against repeated intrathecal injection of 1% ropivacaine in the spinal nerve.

关 键 词：罗哌卡因 蛛网膜下腔 神经营养素-3 神经毒性 

分 类 号：R614[医药卫生—麻醉学]