甲氨蝶呤对大鼠脊髓挫伤急性期脂质过氧化的影响  

Effects of Methotrexate on Acute Phrase of Spinal Cord Contusion-Induced Lipid Peroxidation in Rats

作　　者：张思[1] 顾兵[1] 王烁宇[2] 李华南[2] 张国福[2] 张水印[1] 

机构地区：[1]江西科技师范大学生命科学学院 [2]江西中医药大学附属医院脊柱外科

出　　处：《神经药理学报》2014年第1期11-16,共6页Acta Neuropharmacologica

基　　金：国家自然科学基金项目(No.30960448);江西省自然科学基金项目(No.20114BAB205033);江西省教育厅科技项目(No.GJJ11596;No.GJJ12584)

摘　　要：目的:观察甲氨蝶呤对大鼠脊髓挫伤后脂质过氧化的影响,探讨其急性期抗氧化神经保护机制。方法:采用PinPointTM精密皮质撞击器制备大鼠脊髓挫伤模型,伤后30 min皮下注射(subcutaneous injection,sc)甲氨蝶呤(0.5 mg·kg-1·BW),采用酶联免疫吸附法检测血浆中丙二醛(malondialdehyde,MDA)和8-异前列腺素F2α(8-iso-Prostaglandin F2α,8-iso-PGF2α)的含量以及损伤组织中4-羟基壬烯醛-His加合物(4-hydroxynonenal-His adduct,HNE)的含量。结果:伤后1、3、6、12、24、48、72 h,甲氨蝶呤组MDA、8-iso-PGF2α以及HNE的含量均低于模型组;尤其在伤后6、12 h,甲氨蝶呤组血浆中MDA和脊髓组织中HNE含量均显著低于模型组(P<0.05);在伤后12 h,血浆中8-iso-PGF2α含量也显著低于模型组(P<0.05)。结论:合适剂量的甲氨蝶呤防止脊髓继发性损伤可能与其抑制或减轻脂质过氧化有关。Objective:This study examined the effect of methotrexate on acute phrase of spinal cord contusion-induced lipid peroxidation in rats and the neuroprotective mechanism of its anti-oxidant action. Methods:Rat spinal cord contusion model was prepared by Pinpoint Precision Cortical ImpactorTM apparatus and then methotrexate(0.5mg·kg-1·BW)was subcutaneously administrated at posttraumatic 30 min. ELISA method was used to determine the content of malondialdehyde(MDA)and 8-iso-PGF2αin plasma and 4-hydroxynonenal-His adduct (HNE)in injuried tissue. Results:Posttraumatic 1,3,6,12,24,48,72 h,the contents of MDA, 8-iso-PGF2αand HNE in the methotrexate group were all lower than those in the model group, which achieved statistical significance at posttraumatic 6,12 h(P<0.05). At posttraumatic 12 h, the content of 8-iso-PGF2αin plasma was also significantly lower than that in the model group (P<0.05). Conclusion:The appropriate doses,methotrexate could prevent secondary injury of spinal cord,which may be related to inhibiting or reducing the lipid peroxidation.

关 键 词：甲氨蝶呤 创伤性脊髓损伤 急性期 脂质过氧化 酶联免疫吸附法 

分 类 号：R331[医药卫生—人体生理学] R965.1[医药卫生—基础医学]