RP-HPLC测定金线莲超声-微波协同萃取物中3种活性成分的含量  

Determination of three active component in Anoectochilus roxburghii(Wall) Lindl with ultrasound-microwave combined on extraction by RP-HPLC

作　　者：曹扬远[1] 朱壁洁[1] 王勇 钟添华[1] 黄丽英[1] 

机构地区：[1]福建医科大学药学院,福州350004 [2]福建省食品药品检验所

出　　处：《中国现代药物应用》2007年第5期1-3,共3页Chinese Journal of Modern Drug Application

基　　金：教育部重点实验室资助项目(项目编号:FS06002);香港汉铭科技有限公司资助项目(项目编号:06B001);福建省自然科学基金资助项目(项目编号:2007J0095)

摘　　要：目的建立金线莲中3个黄酮类成分的提取工艺和含量测定方法。方法应用超声-微波协同提取珍稀金线莲药材中的黄酮类成分,正交实验对提取工艺进行了优化;RP-HPLC测定其含量,色谱柱为AgilentC18柱(4.6×250mm,5μm),甲醇-水-磷酸(52∶48∶0.04)为流动相,流速1.0ml/min,检测波长368nm;结果3个黄酮类成分槲皮素、山萘酚、异鼠李素在该色谱条件下获良好分离,线性范围分别为0.10～1.60,0.10～1.60,0.2～3.2μg,线性关系良好(r=0.9995,09996,0.9997),加样回收率分别为98.2%,97.9%,98.4%。测定了不同产地的金线莲药材,槲皮素、山萘酚和异鼠李素含量分别在0.109～0.197mg/g,0.089～0.126mg/g和0.281～0.356mg/g。结论对不同产地的野生金线莲药材中槲皮素、山萘酚和异鼠李素的含量进行系统研究,该法简捷,准确快速。Objective To establish a method for extraction and determination of flavonodis in Anoectochilus roxburghii(Wall)Lindl.Methods The effect of ultrasound-microwave combined on extraction of Anoectochilus roxburghii(Wall)Lindl has been studied.The optimized condition of extraction of flavonoids was studied with orthogonal design.The reversed phase HPLC system consisting of an Agilent ODS column(4.6 mm×250 mm,5 μm)and a mixture of CH3OH-H2O-H3PO4(52 ∶ 48 ∶ 0.4)as the mobile phase was used.The detection wavelength was at 368 nm and the flow rate was at 1.0 ml/min.Results The quercetin,kaempferorl and isorhamnetin were well separated by this method.Linearities of quercetin,kaempferorl and isorhamnetin were good(r=0.999 5,0.999 6,0.999 7)in ranges of 5.0～80.0,5.0～80.0,10.0～160.0 μg,respectively.The average recoveries were 98.2%,97.9%,98.4%.The contents of quercetin,kaempferol and isorhamnetin was from 0.109～0.197 mg/g,0.089～0.126 mg/g and 0.281～0.356 mg/g,groups of Anoectochilus roxburghii(Wall)Lindl from different location.Conclusion The method for extraction and determination is simple,accurate and can be used for the quality study of Anoectochilus roxburghii(Wall)Lindl.

关 键 词：金线莲 超声-微波法 含量测定 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]