镉暴露与金属硫蛋白MT-2A基因多态性和新生儿出生头围的关系  

Effect of Cadmium Exposure and MT-2A Single Nucleotide Polymorphism on Birth Head Circumference of Newborns

作　　者：黄丽华[1] 王君[1] 张云[1] 金银龙[1] 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所

出　　处：《环境卫生学杂志》2014年第2期105-109,共5页JOURNAL OF ENVIRONMENTAL HYGIENE

基　　金：卫生行业科研专项(201002001)

摘　　要：目的探讨镉暴露与金属硫蛋白MT-2Ars 28366003位点基因多态性和新生儿出生头围的关系。方法选取300对产妇与新生儿,对产妇进行问卷调查,新生儿进行出生医学检查。采用电感耦合等离子质谱(ICP/MS)检测产妇血液、脐血中的镉浓度,聚合酶链式反应—限制性片段长度多态性(PCR-RFLP)方法检测产妇血液及脐血金属硫蛋白MT-2A 28366003位点基因多态性。结果①母亲、新生儿中MT-2Ars28366003位点杂合/突变型基因携带者的血镉浓度均高于野生型携带者(P<0.05);②母亲血镉高暴露组的新生儿头围比低暴露组小(P<0.05),新生儿携带杂合型基因组与野生型基因组相比,头围无差异(P>0.05);③母亲镉高暴露、新生儿携带杂合型组的出生头围小于母亲镉低暴露、新生儿携带野生型组(P<0.05),其余各组间无差异。结论 MT-2Ars 28366003位点基因多态性会影响产妇、新生儿血镉的浓度;镉暴露会影响新生儿出生头围,且受MT-2A 28366003位点基因多态性的修饰。Objectives To explore the effect of cadmium exposure and MT-2A single nucleotide polymorphism on birth head circumference of the newborns. Methods 300 pairs of lying-in women-newborns were selected for questionnaire survey,maternal and cord blood of the subjects were collected respectively to measure the concentration of cadmium by ICP/MS,the MT- 2Ars 28366003 single nucleotide Polymorphism were detected by Polymerase chain reaction-restriction fragment length polymorphism. Results ① Statistically significant associations were found between the- 5 A/G core promoter region SNP in the MT2A gene and cadmium levels of lying-in women and newborns.(P < 0. 05). ② The cadmium concentration of mother can influence the birth head circumference(P > 0. 05). ③ In comparison of birth head circumference in four subgroups defined by cadmium concentration group(high/low) and newborn genotype of MT-2A(AA,AG),it was found that mothers in high cadmium exposure,infants carrying the heterozygote genotype(AG) had significantly birth head circumference reduction(P < 0. 05). Conclusions The MT-2Ars 28366003 single nucleotide Polymorphism is associated with blood cadmium concentration; Prenatal cadmium exposure can reduce the head circumference of newborn,and be modified by MT-2Ars 28366003 single nucleotide Polymorphism.

关 键 词：镉 MT-2A基因 单核苷酸多态性 出生头围 

分 类 号：R114[医药卫生—卫生毒理学]