黄药子与甘草配伍对大鼠肝脏CYP450基因表达的影响  

Effects of Compatibility of Rhizoma Dioscoreae Bulbiferae and Radix Glycyrrhizae on Expression of CYP450 mRNA in Rat Liver

作　　者：华碧春[1] 黄智锋[1] 刘娇[1] 程心玲[2] 陈小峰[3] 王英豪[1] 卓实[2] 

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]福建中医药大学附属人民医院,福建福州350004 [3]福建中医药大学附属第二人民医院,福建福州350003

出　　处：《福建中医药大学学报》2014年第1期19-21,共3页Journal of Fujian College of Traditional Chinese Medicine

基　　金：国家自然科学基金资助课题(81274105)

摘　　要：目的研究甘草减轻黄药子肝损伤及对CYP450基因表达的影响。方法采用逆转录聚合酶链反应技术检测大鼠肝脏CYP1A2、CYP2C19的mRNA的表达。结果在mRNA水平上,28、35 d黄药子组显著诱导CYP1A2的基因表达,配伍甘草后表达降低,差异具有统计学意义。结论甘草通过抑制CYP1A2的mRNA表达,对黄药子致肝损伤具有一定的保护作用。Objective To investigate the effects of radix glycyrrhizae on alleviating hepatic injury caused by rhizoma dioscoreae bulbiferae and expression of CYP450 mRNA in rat liver. Methods The mRNA expression of CYP1A2 and CYP2C19 in rat liver were detected by RT-PCR. Results The mRNA expression of CYP1A2 was induced significantly by rhizoma dioscoreae bulbiferae after rats were gastric perfused with drugs for 28 days and 35 days separately, and the expression was decreased significantly after compatibil-ity of radix glycyrrhizae(P<0.05). Conclusion Radix glycyrrhizae has protective effects on liver injury caused by rhizoma dioscoreae bulbiferae by inhibiting the mRNA expression of CYP1A2.

关 键 词：黄药子 甘草 CYP450 基因表达 配伍减毒 

分 类 号：R285.5[医药卫生—中药学]