蛇葡萄素对人乳腺癌MCF-7细胞增殖与凋亡的影响  

Effect of Ampelopsin on Proliferation and Apoptosis of Human Breast Cancer MCF-7 Cells

作　　者：姚欣[1] 倪峰[1,2] 郭丹[1] 周春权[1] 陈晓明[1] 林静瑜[1] 

机构地区：[1]福建省中医药研究院,福建福州350003 [2]福建卫生职业技术学院,福建福州350101

出　　处：《福建中医药大学学报》2014年第1期22-24,共3页Journal of Fujian College of Traditional Chinese Medicine

基　　金：福建省卫生厅科研项目资助课题(WZZE201302)

摘　　要：目的研究蛇葡萄素对人乳腺癌MCF-7细胞增殖与凋亡的影响。方法用MTT比色法检测蛇葡萄素对MCF-7细胞的增殖抑制作用,绘制量效曲线并计算IC50,流式Annexin V/PI双染法检测细胞凋亡率。结果蛇葡萄素能明显抑制MCF-7细胞增殖,IC50为19.15μg/mL;蛇葡萄素对MCF-7细胞作用6 h后,细胞凋亡率显著增加,且呈量效关系。结论蛇葡萄素能显著抑制MCF-7细胞的增殖,诱导细胞凋亡。Objective To observe the effect of ampelopsin on proliferation and apoptosis of human breast cancer MCF-7 cells. Methods The inhibitory effect of ampelopsin on proliferation of MCF-7 cells was detected by MTT colorimetricassay, the dose-response curve was drawn, the IC50was calculated, and the apoptotic rate was detected by Annexin V / PI double-staining method. Results Ampelopsin inhibited the proliferation of MCF-7 cells, the IC50was 19.15 μg / mL. After the MCF-7 cells were treated with ampelopsin for 6 hours, the apoptotic rate was increased significantly, and does-effect relationship existed in the both two sides. Conclusion Ampelopsin can inhibit the proliferation of MCF-7 cells significantly, and induce the apoptosis.

关 键 词：蛇葡萄素 乳腺癌 MCF-7 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]