石斛合剂对2型糖尿病模型大鼠脂肪细胞因子基因表达的影响  

Effect of Dendrobium Compound on Adipocytokine Gene Expression in Type 2 Diabetic Model Rats

作　　者：王林[1] 严晓丹[1] 王晓宁[1] 刘洪波[1] 施红[1] 

机构地区：[1]福建中医药大学中西医结合学院,福建福州350122

出　　处：《福建中医药大学学报》2014年第3期8-1,共3页Journal of Fujian College of Traditional Chinese Medicine

基　　金：国家自然科学基金资助课题(81072736);福建省自然科学基金资助课题(2012J5153)

摘　　要：目的观察石斛合剂对2型糖尿病模型大鼠脂肪细胞因子基因表达的影响。方法对2型糖尿病模型大鼠用石斛合剂灌胃,用荧光实时定量PCR法检测大鼠脂肪组织中内脂素(Visfatin)、丝氨酸蛋白酶抑制剂(Vaspin)、抵抗素(Resistin)、白细胞介素-6(IL-6)基因mRNA的表达。结果 2型糖尿病模型大鼠Vaspin表达升高,Visfatin、Resistin、IL-6表达降低。结论石斛合剂可改善2型糖尿病模型大鼠脂肪细胞因子的表达,具有多途径多靶点的抗炎作用。Objective To observe the effect of Dendrobium compound on adipocytokine gene expression in type 2 diabetic model rats. Methods Type 2 diabetic model rats were gastric perfused with Dendrobium compound, and the gene expressions of Visfatin, serine proteinase inhibitors such as Vaspin, Resistin and interleukin-6(IL-6) in adipose tissue of rats were detected by fluorescent quantitation PCR(FQ-PCR) assay. Results The expression of Vaspin in Type 2 diabetic model rats received Dendrobium compound by gastric perfusion was increased, while Vaspin, Resistin and IL-6 were significantly decreased.Conclusion Dendrobium compound can improve the expression of adipocytokine expression in type 2 diabetic model rats, and has anti-inflammatory effects of multiple ways and multiple targets.

关 键 词：糖尿病 脂肪细胞因子 内脂素 丝氨酸蛋白酶抑制剂 抵抗素 白细胞介素-6 大鼠模型 

分 类 号：R285.5[医药卫生—中药学]