针灸对乌头碱致心律失常家兔心脏M_3受体mRNA表达的影响  

Effects of Acupuncture and Moxibustion on mRNA Expression of M_3 Receptor in Heart of Cardiac Arrhythmia Rabbits Caused by Aconitine

作　　者：郑淑霞[1] 许金森[1] 潘晓华[1] 陈凌[1] 兰彩莲[1] 

机构地区：[1]福建省中医药研究院,福建福州350003

出　　处：《福建中医药大学学报》2014年第3期19-21,共3页Journal of Fujian College of Traditional Chinese Medicine

基　　金：国家自然科学青年基金资助课题(81001505);福建省卫生厅中医药科研一般课题(WZZH201301);福建省中医药研究院自主选题(2012FJZYYK-6)

摘　　要：目的探讨针刺和艾灸内关穴对心律失常的调节机制。方法新西兰家兔40只,随机分成对照组、药物组、针刺内关穴组、艾灸组,用半定量逆转录聚合酶链反应(RT-PCR)法检测4组家兔心肌组织中M3受体mRNA的相对含量。结果药物组心肌M3受体mRNA的相对含量较对照组高;电针组心肌M3受体mRNA的相对含量较药物组低;艾灸组心肌M3受体mRNA的相对含量较药物组低,3组差异有统计学意义(P<0.05)。结论乌头碱诱发心律失常后,心肌M3受体mRNA的表达增强,电针和艾灸可在一定的程度上减少心肌M3受体mRNA的表达。Objective To investigate the regulatory mechanism of applying acupuncture and moxibustion on Neiguan(PC 6) in treating cardiac arrhythmia. Methods 40 New Zealand rabbits were randomly divided into control group, drug group, acupuncture group and moxibustion group. The mRNA expression of M3 receptor in heart muscle tissue of each group was detected by RT-PCR. Results The mRNA expression of M3 receptor in drug group was higher than that of control group, the index in acupuncture group was lower than that of drug group, the index in moxibustion group was lower than that of drug group,and there were significant differences among the three groups(P <0.05). Conclusion After cardiac arrhythmia has been induced by aconitine, the mRNA expression of M3 receptor is increased; when acupuncture or moxibustion, the index is deereased, which suggests acupuncture and moxibustion can cure cardiac arrhythmia by down-regulating the mRNA expression of M3 receptor.

关 键 词：心律失常 M3受体 逆转录聚合酶链反应 电针 艾灸 

分 类 号：R245[医药卫生—针灸推拿学]