筋骨草总黄酮对大鼠肝星状细胞MMP-13和TIMP-1表达的影响  

Effect of Total Flavonoids from Ajuga Decumbens on Expressions of MMP-13 and TIMP-1 in Hepatic Stellate Cells of Rats

作　　者：赖文芳[1] 吴符火[1] 南丽红[1] 

机构地区：[1]福建中医药大学药学院,福建福州350122

出　　处：《福建中医药大学学报》2014年第3期22-24,共3页Journal of Fujian College of Traditional Chinese Medicine

基　　金：陈可冀中西医结合发展基金资助课题(CKJ2007026)

摘　　要：目的观察筋骨草总黄酮对大鼠肝星状细胞(HSC)增殖、MMP-13、TIMP-1表达的影响。方法用筋骨草总黄酮含药血清对HSC细胞进行干预,分为空白对照组、5%、10%、20%3个含药血清组,用MTT检测HSC细胞增殖,ELISA检测Ⅰ型胶原浓度,RT-PCR检测MMP-13、TIMP-1 mRNA的表达。结果与空白对照组比较,含药血清3个剂量组对HSC细胞的抑制作用增强,I型胶原浓度降低,促进MMP-13、抑制TIMP-1 mRNA表达,随着药物浓度增加,MMP-13/TIMP-1比值增大,与I型胶原呈负相关。结论筋骨草总黄酮抗肝纤维化的机制是抑制HSC增殖,促进MMP-13、抑制TIMP-1mRNA表达,促进Ⅰ型胶原降解,抑制细胞外基质积聚。Objective To observe the effect of total flavonoids from ajuga decumbens(TFAD) on the proliferation of rat hepatic stellate cells(HSC), and the expressions of matric metalloproteinase-13(MMP-13) and tissue inhibitor of metalloproteinase-1(TIMP-1). Methods The HSC were treated with TFAD drug-containing serum, then the cells were divided into blank control group, 5% TFAD drug-containing serum group, 10% TFAD drug-containing serum group and 20% TFAD drug-containing serum group. The HSC proliferation was detected by MMT assay, the content of collagen-I was detected by ELISA assay, and the expressions of MMP-13 and TIMP-1 mRNA were detected by RT-PCR. Results Compared with blank control group, the HSC in TFAD drug-containing serum groups were inhibited significantly, the content of collagen-I was decreased respectively, the expression of MMP-13 mRNA was increased respectively, and TIMP-1 mRNA was decreased respectively. With the increase of drug concentration, the MMP-13 / TIMP-1 ratio was increased, which was significantly negative correlation with collagen-I. Conclusion TFAD has the effect of anti-hepatic fibrosis, and the possible mechanism may be related with inhibiting HSC proliferation, promoting MMP-13 mRNA expression, inhibiting TIMP-1 mRNA expression, then promoting degradation of collagen-I,and decreasing extracellular matrix.

关 键 词：肝纤维化 筋骨草总黄酮 肝星状细胞 大鼠模型 

分 类 号：R285.5[医药卫生—中药学]