中部太平洋大眼金枪鱼(Thunnus obesus)种群遗传结构的初步研究  

POPULATION GENETIC STRUCTURE OF THUNNUS OBESUS IN CENTRAL PACIFIC OCEAN

作　　者：吴智超[1] 许强华[1,2,3,4] 许柳雄[1,2,3,4] 戴小杰 朱江峰[1,2,3,4] 

机构地区：[1]上海海洋大学海洋科学学院,上海201306 [2]大洋渔业资源可持续开发省部共建教育部重点实验室,上海201306 [3]农业部大洋渔业资源环境科学观测实验站,上海201306 [4]国家远洋渔业工程技术研究中心,上海201306

出　　处：《海洋与湖沼》2014年第1期60-65,共6页Oceanologia Et Limnologia Sinica

基　　金：上海市捕捞学重点学科专项基金项目;S30702号;国家863计划;2012AA092303号

摘　　要：大眼金枪鱼(Thunnus obesus)是世界最重要的渔业资源之一,经济价值较高,捕捞压力的加大使中部太平洋大眼金枪鱼过度捕捞程度愈发严重。开展种群遗传结构研究将为大眼金枪鱼资源的可持续利用与开发提供科学依据。本文以取自中西太平洋和中东太平洋共182尾大眼金枪鱼个体为研究对象,分析研究其线粒体DNA控制区第一个高变区(the first hypervariable region,HVR-1)457bp的序列,共发现115个变异位点,定义了178种单倍型。序列多样性分析结果显示:8个采样点核苷酸多样性在0.03074—0.04362之间,单倍型多样性在各个采样点都较高,变动范围在0.996—1.000之间;分子方差分析显示99.97%的遗传变异性来自于种群内;群体间的高基因交流值Nm和低分化指数Fst揭示中东太平洋与中西太平洋群体间基因交流频繁,不存在显著的遗传分化。Overexploitation of bigeye tuna in the Central Pacific Ocean has become a serious concern. We investigated the population genetic structure aiming at providing scientific references for the sustainable development of the resources. In this study, 182 bigeye tuna specimens were sampled from the Central Pacific Ocean. Population genetic diversity was evaluated by using the sequence data of the first HVR-1(hypervariable region, HVR-1) of mitochondrial control region. In total, 115 variable sites were observed and 178 haplotypes were identified. Analysis of mtDNA HVR-1 sequences of bigeye tuna from eight localities with the nucleotide diversities ranged 0.03074-0.04362 and high haplotype diversities ranged 0.996-1.000. AMOVA test of bigeye tuna revealed that 99.97% of the genetic variation occurred within populations. Low Fst(fixation index) values and high gene flow(Nm) values revealed in this investigation indicate no significant genetic differentiation and high rates of gene flow between the Central Western Pacific bigeye tuna populations and the Central Eastern Pacific bigeye tuna populations.

关 键 词：大眼金枪鱼(Thunnus obesus) 种群遗传结构 线粒体DNA 控制区 

分 类 号：S917.4[农业科学—水产科学]