小鼠Th17细胞分化模型的构建  

Construction of mouse Th17 cell differentiation model

作　　者：陈嵘祎[1] 周飞[1] 周英[1] 阿彩岭[1] 王丹纯[1] 

机构地区：[1]广东医学院附属医院皮肤科,广东湛江524001

出　　处：《广东医学院学报》2014年第1期14-16,19,共4页Journal of Guangdong Medical College

基　　金：国家自然科学青年基金(No.GZ1112);2012年广东省大学生创新项目(No.Ky1211);广东医学院博士启动项目(No.XB1022);广东医学院附属医院博士启动项目(No.11001B01002)

摘　　要：目的构建小鼠Th17细胞体外诱导分化模型。方法提取小鼠脾淋巴细胞,采用免疫磁珠纯化++CD4 CD62L T细胞(原始T细胞),分对照组及Th17组。Th17组采用CD3、CD28、IFN-、IL-4抗体及IL-6、TGF-1、IL-23培养72 h,采用Real-time PCR检测RORt和IL-17F mRNA表达。结果 Th17组T细胞RORt、IL-17F mRNA表达明显高于对照组(P<0.01)。结论构建Th17细胞体外诱导分化模型可用于T细胞分化研究。Objective To construct an in vitro Th17 cell differentiation model in mice. Methods Mouse spleen + +lymphocyt-es were purified by using CD4 CD62L T cell isolation kit II, and then divided into control and Th17 groups. T cells were cultured with anti-mouse CD3, CD28, IFN-, and IL-4 antibodies, and IL-6, TGF-a1, and IL-23 for 72 hours. After harvesting, RORt and IL-17F mRNA expression were detected by real-time PCR. Results RORt and IL-17 F mRNA expression was upregulated in Th17 group compared with control group(P<0.01). Conclusion Construction of the in vitro Th17 cell differentiation model can be feasible in the study of T cell differentiation.

关 键 词：T细胞 分化 TH17细胞 模型 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]