雌激素受体-α、β在雌性山羊结状神经节的分布  

Distribution of ER-α and ER-β in Nodose Ganglion of Female Goat

作　　者：董伟[1] 徐永平[1] 金秀芳[1] 李强[1] 陈文东[1] 郭晓[1] 

机构地区：[1]西北农林科技大学动物医学院,杨凌712100

出　　处：《畜牧兽医学报》2014年第5期809-814,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(31072184);陕西省农业推广项目(k332021309)

摘　　要：探究结状神经节内脏感觉神经元是否是雌激素作用的靶细胞。采用免疫组织化学SP法观察雌激素受体(ER-α、β)在成年雌性山羊结状神经节内的分布特点。结果显示:ER-α和ER-β免疫反应阳性物质主要分布在神经元中,其所有神经元细胞核呈棕黄色,为强阳性;而大型神经元细胞质呈黄色,为中等阳性;中小型神经元细胞质呈棕黄色,为强阳性。在卫星细胞中,ER-α、β免疫反应阳性物质呈黄色,为中等阳性。图像分析表明,神经元上ER-α、β的相对表达量结果与神经节内其它成分相比,差异极显著(P<0.01)。结果证明ER-α、β在结状神经节中表达,表明结状神经节内脏感觉神经元是雌激素发挥作用的主要靶细胞之一。The aim of this study was to explore whether the visceral sensory neurons in Nodose Ganglion(NG)were the major target cells of estrogen.Immunohistochemical SP method was adopted to observe the distribution of estrogen receptor(ER-α,β)in NG of female goats.The results indicated that all the nucleus of neurons was strong positive stained with brown to ER-α,β. While the cytoplasm of large-sized neurons showed medium positive with yellow and the cytoplasm of small and medium-sized neurons showed strong positive with claybank.In satellite cells, their medium positive expression was observed.Image analysis showed a significant difference of ER expression between neurons and non-neurons(P<0.01).The results showed that ER-α,β were expressed in NG,which indicate visceral sensory neurons in NG were the main target cells of estrogen.

关 键 词：雌激素受体 结状神经节 免疫组织化学SP法 雌性山羊 

分 类 号：S858.27[农业科学—临床兽医学]