青蒿素对鸡柔嫩艾美耳球虫第二代裂殖子微线基因mRNA转录及鸡盲肠组织结构的影响  

Effects of Artemisinin Treatment on Microneme Genes Transcription in Second-generation Merozoites and Pathological Changes of Caecum in Chickens Infected by Eimeria tenella

作　　者：莫平华[1,2] 马庆涛[1,2] 纪小霞 宋萍[1,2] 陶建平 李金贵[1,2] 

机构地区：[1]扬州大学兽医学院,扬州225009 [2]江苏省动物重要疫病与人畜共患病防控协同创新中心,扬州225009

出　　处：《畜牧兽医学报》2014年第5期833-838,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：江苏省高校优势学科建设工程资助项目;国家自然科学基金资助项目(31272548)

摘　　要：拟探讨青蒿素抗鸡柔嫩艾美耳球虫(Eimeria tenella)的作用机制。将试验鸡随机分为空白对照组、攻毒对照组和青蒿素药物组,于攻毒后120h,收集试验鸡的盲肠并提取E.tenella第二代裂殖子。通过红细胞计数法计算第二代裂殖子数量;SYBR Green I荧光定量PCR检测第二代裂殖子微线基因(EtMIC1、EtMIC2、EtMIC3、EtMIC4、EtMIC5)mRNA相对转录量及扫描电镜观察试验鸡盲肠组织的病理学变化。结果表明:与攻毒对照组比较,青蒿素组的球虫第二代裂殖子数量降低了25.37%(P<0.05),同时裂殖子中微线基因EtMIC1、EtMIC2、EtMIC4的mRNA转录水平均显著下调(P<0.05),EtMIC3、EtMIC5则极显著下调(P<0.01);青蒿素组盲肠的病理组织结构也得到改善。青蒿素减轻鸡盲肠组织病变可能与其下调EtMICs mRNA转录并使裂殖子数量减少有关。The aim of this study was to study the mechanism of artemisinin against E.tenella.At 120hafter inoculation by oral E.tenella sporulated oocysts,we collected the second generation merozoites of E.tenellafrom the chicken's caecum in different groups(challenge control group, and artemisinin-treated group)and counted with erythrocytometer.mRNA transcription of microneme genes(EtMIC1,EtMIC2,EtMIC3,EtMIC4,EtMIC5)in second-generation merozoites were analyzed using quantitative real-time PCR.The pathological structural changes of chicken cecal tissue were observed by scanning electron microscopy.Compared with the challenge control group,the number of second generation merozoites of E.tenella was decreased by 25.37%(P< 0.05)in artemisinin-treated group.Meanwhile,the transcription level of EtMIC1,EtMIC2,EtMIC3,EtMIC4,EtMIC5were decreased by 46.59%(P<0.05),36.53%(P<0.05),57.89%(P<0.01),38.09%(P<0.05),54.53%(P<0.01),respectively.The cecal pathological structures were partially improved by artemisinin treatment.These results suggested that artemisinin may decrease the number of merozoites by inhibiting the expression levels of microneme genes insecond-generation merozoites,and then result in alleviating the cecal lesion.

关 键 词：鸡 青蒿素 柔嫩艾美尔球虫 盲肠 微线基因 荧光定量PCR 

分 类 号：S858.31[农业科学—临床兽医学]