九龙牦牛肌细胞生成素基因的克隆及其表达谱  

Cloning and Expression Profiling of Myogenin Gene of Jiulong Yak

作　　者：林亚秋[1] 张倡珲[1] 郑玉才[1] 王永[1] 黄林[1] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041

出　　处：《畜牧兽医学报》2014年第2期207-211,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家民委项目(12XNZ002);西南民族大学中央高校基本科研业务费专项(13NZYQN23)

摘　　要：旨在获得九龙牦牛(Bos grunniens)肌细胞生成素(Myogenin,MyoG)基因序列并进行生物信息学分析,同时分析其组织表达谱和时序表达谱,为进一步研究九龙牦牛肌肉发育和肉质形成奠定基础。本试验通过RT-PCR技术克隆九龙牦牛MyoG基因,利用半定量RT-PCR技术检测该基因在九龙牦牛不同组织中的表达情况,利用荧光定量PCR技术检测该基因在九龙牦牛不同发育时期背最长肌的表达情况。结果表明,获得九龙牦牛700bp的MyoG基因序列(GenBank登录号:KC184120),其中ORF为675bp,编码224个氨基酸,与普通牛、人、小鼠、大鼠、猪、羊和兔MyoG氨基酸序列同源性为92%~99%;MyoG基因仅在背最长肌中检测到表达,在心、肝、脾、肾和脂肪中未检测到表达;MyoG基因随着九龙牦牛年龄段增长表达呈上升趋势,且在3.5~5.5和9岁以上的牛肌背最长肌中的表达显著高于0.5岁牛的表达水平(P【0.05)。结果表明,MyoG基因可能在九龙牦牛肌肉组织发育过程中发挥重要的调控作用。The aim of the present study was to clone myogenin(MyoG)gene of Jiulong yak,to carry out bioinformatics analysis and to assay gene expression profiling in different tissues and developmental stages,in order to lay the foundation for studing meat quality formation and muscle development of Jiulong yak.Based on the published nucleotide sequence of bovine MyoGgene in GenBank,apair of primer was designed.The Jiulong yak MyoGgene was amplified by RT-PCR, the tissue specificity of MyoGgene were analyzed by semi-quantitative RT-PCR,and different development stages expression specificity of MyoGgene were analyzed by fluorescence quantitative PCR.The results showed that Jiulong yak MyoGgene cDNA was 700bp(GenBank accession number:KC184120),with an ORF of 675bp encoding 224amino acids.Compared with cattle,human,mouse,rat,pigs,sheep,rabbit,the homology of amino acid sequences were 92%-99%.The MyoGgene expression was only observed in longissimus muscle,and not detected in heart,liver, kidney,spleen and fat.The MyoG mRNA expression level increased with Jiulong yak growth, showing higher mRNA level in longissimus muscle of 3.5-5.5and over 9years old Jiulong yaks than that in 0.5years old Jiulong yaks(P<0.05).These results suggest that MyoG may play an important role in the development of muscle tissue of Jiulong yak.

关 键 词：九龙牦牛 MYOG 克隆 基因表达 

分 类 号：S823.85[农业科学—畜牧学]