脂肪组织特异性PU.1敲除小鼠的鉴定及其活体脂肪和肌肉含量分析  

Identification of Adipose-specific PU.1 Knockout Mice and Analysis of Their Live Body Fat and Lean Contents

作　　者：庞卫军[1,2] 卫宁[1] 王禹[1] 熊燕[1] 童强 杨公社[1] 

机构地区：[1]西北农林科技大学动物脂肪沉积与肌肉发育实验室,杨凌712100 [2]贝勒医学院,美国休斯顿77030

出　　处：《畜牧兽医学报》2014年第2期225-232,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家"973"计划子项目(2012CB124705);国家自然科学基金(30600437);西北农林科技大学基本科研业务项目(QN2009021);国家生猪产业技术体系(CARS-36)

摘　　要：本研究运用基因工程和同源重组等技术,产生了脂肪组织特异性PU.1敲除小鼠(aP2-cre-PU.1fl/fl),并用PCR技术进行基因型判定。Western blot结果表明,与PU.1fl/fl小鼠相比,aP2-cre-PU.1fl/fl小鼠脂肪组织PU.1蛋白表达显著降低,达到敲除水平。在此基础上,对1~6月龄的PU.1fl/fl和aP2-cre-PU.1fl/fl小鼠体重和活体成分进行了分析。研究发现,aP2-cre-PU.1fl/fl小鼠在出生后第5和第6月龄体重显著高于PU.1fl/fl小鼠(P【0.05);6月龄时,肌肉量无显著差异,但aP2-cre-PU.1fl/fl小鼠脂肪量明显高于PU.1fl/fl小鼠(P【0.05)。结果提示,aP2-cre-PU.1fl/fl小鼠脂肪重量的增加是其体重增加的主要原因。脂肪组织特异性PU.1敲除小鼠的生产为深入研究PU.1基因在体调控脂肪生成的功能奠定了基础,也为探索PU.1基因控制家畜体脂沉积提供了理论依据。aP2-cre-PU.1fl/fl mice were generated and identified using gene engineering,homologous recombination,PCR and Western blotting technologies in this study.The results showed that,compared with PU.1fl/fl mice,PU.1protein was down-regulated to the level of that of aP2-cre-PU.1fl/fl.The body weight and body composition were analyzed in PU.1fl/fl and aP2-cre-PU. 1fl/fl mice with 1-6month old.Moreover,the body weight of aP2-cre-PU.1fl/fl mice was significantly higher than that of PU.1fl/fl mice at month 5and month 6(P<0.05).There was no significant difference in muscle weight but fat weight was significantly higher in aP2-cre-PU.1fl/fl mice than that in PU.1fl/fl mice(P<0.05).The results suggest that increase of adipose tissue of aP2-cre-PU.1fl/fl mice results in the increase of their body weight.Therefore,aP2-cre-PU.1fl/fl mice is the better animal model to explore PU.1physiological functions in adipose tissue,which provide the theorical basis for exploring the effect of PU.1gene on fat deposition in animals.

关 键 词：PU.1 脂肪组织特异性敲除小鼠 脂肪 肌肉 活体成分分析 

分 类 号：R589.2[医药卫生—内分泌]