鸽源新城疫病毒全基因组序列分析及致病性研究  

Complete Genome Sequence Analysis and Pathogenicity of Newcastle Disease Virus Isolated from Pigeons

作　　者：胡北侠[1] 杨少华[1] 张会娟[2] 伊惠[2] 张伟[2] 王莉莉 许传田[1] 张琳[1] 黄庆华[1] 张秀美[1] 

机构地区：[1]山东省农业科学院畜牧兽医研究所山东省畜禽疫病防治与繁育重点实验室,济南250100 [2]山东农业大学动物科技学院,泰安271018 [3]山东省微山县畜牧局,微山277600

出　　处：《畜牧兽医学报》2014年第2期255-261,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：公益性行业(农业)科研专项(201003012;201303033);现代农业产业技术体系建设专项资金(CARS-42-Z12)

摘　　要：为了科学理解新城疫病毒(NDV)的遗传进化规律,对鸽源NDV基因组和致病特征进行了鉴定分析。从发病鸽群中分离鉴定1株NDV,命名为Pigeon/China/SDLC/2011,并对其进行全基因组测序分析和致病性评价。测序结果显示Pigeon/China/SDLC/2011基因组为15 192bp,具有NDV强毒株F蛋白裂解位点112 RRQKRF117。遗传进化分析发现Pigeon/China/SDLC/2011属于基因Ⅵ型,其6个结构蛋白氨基酸序列与基因Ⅶ型NDV参考株相比存在不同程度变异,其中P和HN蛋白相似性最低,分别为84.6%~84.8%和90.0%~91.1%。致病性试验结果显示鸽感染后表现瘫痪、翅膀下垂或斜颈等神经症状和排绿色稀粪,病死率为70%~80%;而SPF鸡感染后没有明显的临床症状。试验鸽喉头和泄殖腔病毒检出率明显高于试验鸡。本研究结果显示Pigeon/China/SDLC/2011在分子特性和对SPF鸡致病性方面与基因Ⅶ型NDV存在明显差异。虽然SPF鸡感染Pigeon/China/SDLC/2011后没有发病死亡,但可向体外排毒,因此必须采取有效措施防止鸽源NDV在鸽群和鸡群之间的相互传播。The genome and pathogenicity of pigeon NDV isolate were characterized for better understanding the evolution of NDV.A strain of Newcastle disease virus,named Pigeon/China/ SDLC/2011,was isolated and identified from diseased pigeon flock.The full-length genome of Pigeon/China/SDLC/2011was sequenced and compared with reference strains.Besides,the pathogenicity of Pigeon/China/SDLC/2011was evaluated in pigeons and SPF chickens.Sequence analysis showed that the complete genome of Pigeon/China/SDLC/2011contained 15 192nucleotides(nt).The cleavage site of the fusion protein was112 RRQKRF117,a feature generally associated with virulent NDV strains.Phylogenetic analysis,based on the genomic sequences and sequences of the fusion gene,revealed that Pigeon/China/SDLC/2011should be classified as genotype Ⅵ NDV. Mutations were observed in the six structural proteins of Pigeon/China/SDLC/2011compared with reference NDV strains of genotypeⅦ.More mutations were localized in the P and HN pro-tein than the other proteins and the amino acid sequence identities of P and HN proteins between Pigeon/China/SDLC/2011and genotypeⅦ NDVs was 84.6%-84.8%,90.0%-91.1%,respectively.In the challenge test,70%-80% of pigeons inoculated with Pigeon/China/SDLC/2011showed a series of nervous disorders:bilateral or unilateral locomotor disturbances of wings or legs,torticollis,and watery green diarrhea and died in 6-14days.However,chickens remained clinically normal throughout the study.Viruses were detected more frequently in the swabs of pigeons than chickens.The results showed that marked differences in molecular characterization and pathogenicity in chickens were recorded between Pigeon/China/SDLC/2011and the virulent NDVs of genotypeⅦ.Although Pigeon/China/SDLC/2011was nopathogenic for chickens,some measures should be taken to prevent virus transmission between pigeions and chickens as viruses were detected in swabs of infected chickens.

关 键 词：鸽 SPF鸡 新城疫病毒 序列分析 致病性 

分 类 号：S852.65[农业科学—基础兽医学]